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. 2017 May 4;22(18):30529.
doi: 10.2807/1560-7917.ES.2017.22.18.30529.

Monitoring influenza virus susceptibility to oseltamivir using a new rapid assay, iART

Larisa V Gubareva  1 , Eric Fallows  2 , Vasiliy P Mishin  1 , Erin Hodges  1 , Abdullah Brooks  3   4 , John Barnes  1 , Alicia M Fry  1 , William Kramp  5 , Roxanne Shively  5 , David E Wentworth  1 , Kristin Weidemaier  2 , Ross Jacobson  2
Affiliations

Monitoring influenza virus susceptibility to oseltamivir using a new rapid assay, iART

Larisa V Gubareva et al. Euro Surveill. .

Abstract

A new rapid assay for detecting oseltamivir resistance in influenza virus, iART, was used to test 149 clinical specimens. Results were obtained for 132, with iART indicating 41 as 'resistant'. For these, sequence analysis found known and suspected markers of oseltamivir resistance, while no such markers were detected for the remaining 91 samples. Viruses isolated from the 41 specimens showed reduced or highly reduced inhibition by neuraminidase inhibition assay. iART may facilitate broader antiviral resistance testing.

Keywords: air-borne infections; influenza virus; surveillance; viral infections.

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Conflict of interest statement

Conflict of interest: EF, KW and RJ are employees of Becton Dickinson.

Figures

Figure
Figure
(A) Workflow of iART testing; (B) Prototype device and kit
See this image and copyright information in PMC

References

    1. Oh DY, Hurt AC. A Review of the Antiviral Susceptibility of Human and Avian Influenza Viruses over the Last Decade. Scientifica (Cairo). 2014;2014:430629. 10.1155/2014/430629 - DOI - PMC - PubMed
    1. Samson M, Pizzorno A, Abed Y, Boivin G. Influenza virus resistance to neuraminidase inhibitors. Antiviral Res. 2013;98(2):174-85. 10.1016/j.antiviral.2013.03.014 - DOI - PubMed
    1. Takashita E, Kiso M, Fujisaki S, Yokoyama M, Nakamura K, Shirakura M, et al. Characterization of a large cluster of influenza A(H1N1)pdm09 viruses cross-resistant to oseltamivir and peramivir during the 2013-2014 influenza season in Japan. Antimicrob Agents Chemother. 2015;59(5):2607-17. 10.1128/AAC.04836-14 - DOI - PMC - PubMed
    1. Okomo-Adhiambo M, Fry AM, Su S, Nguyen HT, Elal AA, Negron E, et al. 2013–14 US Influenza Antiviral Working Group Oseltamivir-resistant influenza A(H1N1)pdm09 viruses, United States, 2013-14. Emerg Infect Dis. 2015;21(1):136-41. 10.3201/eid2101.141006 - DOI - PMC - PubMed
    1. Hurt AC, Hardie K, Wilson NJ, Deng YM, Osbourn M, Leang SK, et al. Characteristics of a widespread community cluster of H275Y oseltamivir-resistant A(H1N1)pdm09 influenza in Australia. J Infect Dis. 2012;206(2):148-57. 10.1093/infdis/jis337 - DOI - PMC - PubMed

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