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. 2017 Winter;16(1):357-365.

Homoisoflavonoids and the Antioxidant Activity of Ophiopogon japonicus Root

Yancui Wang  1 , Feng Liu  2   3 , Zongsuo Liang  1 , Liang Peng  1 , Bangqing Wang  1 , Jing Yu  1 , Yingying Su  4 , Cunde Ma  4
Affiliations

Homoisoflavonoids and the Antioxidant Activity of Ophiopogon japonicus Root

Yancui Wang et al. Iran J Pharm Res. 2017 Winter.

Abstract

The root of Ophiopogon japonicus has been used as a traditional Chinese medicine and also a functional food ingredient for a long time in China. In the present study, 17 different homoisoflavonoid compounds were identified in the root extract of O. japonicus by HPLC-DAD and LCMS/MS analyses. The antioxidant activity of the of chloroform/methanol (1:1, v/v), methanol and 70% ethanol extracts, and two major isolated homoisoflavonoid compounds (methylophiopogonanone A and methylophiopogonanone B) from O. japonicus root were investigated by various in-vitro assays. Methylophiopogonanone B showed the highest antioxidant ability according to four antioxidant methods. Among the extracts, the chloroform/methanol extract which contained high amounts of homoisoflavonoids was found to exhibit the strongest antioxidant activity. The results showed that O. japonicus root can be regarded as a potential source of homoisoflavonoids and natural antioxidant.

Keywords: Antioxidant activity; Homoisoflavonoids; LCMS/MS analysis; Ophiopogon japonicas.

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Figures

Figure 1
Figure 1
HPLC chromatogram of (A) chloroform/methanol extract, (B) methanol extract, and (C) 70% ethanol extract of O. japonicus root. Peak 1: methylophiopogonanone A; peak 2: methylophiopo- gonanone B
Figure 2
Figure 2
Chemical structures of methylophiopogonanone A and methylophiopogonanone B
Figure 3
Figure 3
LCMS/MS total ion current profile in negative ion mode for the chloroform/methanol extract of O. japonicus root. The peak numbers show the homoisoflavonoids that are identified in Table 1
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