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. 2017 Jul;34(7):905-911.
doi: 10.1007/s10815-017-0937-z. Epub 2017 May 11.

Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS

S Mateo  1 , F Vidal  2 , M Parriego  3 , I Rodríguez  4 , V Montalvo  3 , A Veiga  3   5 , M Boada  3
Affiliations

Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS

S Mateo et al. J Assist Reprod Genet. 2017 Jul.

Abstract

Purpose: The purpose of this study was to investigate the chromosomal constitution and the developmental potential of intracytoplasmic sperm injection (ICSI) deriving embryos displaying a single pronucleus at the zygote stage.

Methods: Eighty-eight embryos from single pronucleus (1PN) two polar bodies (2PB) ICSI zygotes from 64 preimplantational genetic screening (PGS) cycles (October 2012-December 2014), were retrospectively analyzed. Zygotes were cultured in a time-lapse incubator. Embryo biopsy was performed on day 3 and genetic analysis approached by array comparative genomic hybridization.

Results: Chromosomal analysis revealed that 17% (15/88) of embryos derived from 1PN 2PB zygotes were diagnosed as euploid. After blastomere biopsy at day 3, the blastocyst rate at day 5 was 3.4% (3/88). Only 2.3% (2/88) euploid blastocysts were obtained. In two couples and after counseling and patient agreement, the transfer of a euploid blastocyst from a 1PN 2PB ICSI zygote was performed resulting in the birth of a healthy child.

Conclusions: These results open the possibility to consider embryos coming from 1PN 2PB ICSI zygotes for transfer when no other embryos from 2PN 2PB ICSI zygotes are available and if a PGS diagnosis of euploidy is obtained. Confirmation of biparental inheritance is strongly recommended.

Keywords: 1PN; Monopronucleated zygotes; PGS; Time-lapse; aCGH.

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Conflict of interest statement

Funding

This work was performed under the auspices of “Càtedra d’Investigació en Obstetrícia i Ginecologia” of the Department of Obstetrics, Gynecology and Reproduction, Dexeus Women’s Health (Barcelona).

Conflict of interest

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Time-lapse imaging of second polar body (PB) extrusion and pronuclear (PN) formation and fading of the 1PN 2PB ICSI zygote that produced an ongoing pregnancy and the delivery of a healthy child. The time of image captures (hours post ICSI) is shown at the bottom left of each image. a. First PB. b Extrusion of the second PB. c–e Formation of 1PN. f Fading of PN
Fig. 2
Fig. 2
Time-lapse imaging of in vitro development until day 3 of 1PN 2PB ICSI zygote that produced an ongoing pregnancy and the delivery of a healthy child. The time of image captures (hours post ICSI) is showed at the bottom left of each image. a First and second PB. b 1PN 2PB. c–f 2-, 4-, 5-, and 8-cell embryo
See this image and copyright information in PMC

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