Bioremediation of phenol in soil through using a mobile plant-endophyte system

Abstract

Plant-endophyte remediation of volatile pollutants in soil is an emerging technology. For more efficient application, plant-endophyte systems were formed through stimulation of transfer of degradative plasmids in plant tissue by co-inoculation of corn, wheat or tomato seedlings with Pseudomonas fluorescens TP13 carrying a self-transmissible degradative plasmid, and P. fluorescens streptomycin-resistant P13 strain. The corn-TP13-P13 (CTP) system had higher degradation activity than other plant-endophyte systems. Transplanting the CTP, from loam to sandy clay loam soil, from greenhouse to field trials, almost completely removed phenol from contaminated soils in 15 d. Intact transplantation of the CTP to contaminated soils was more efficient than co-transplanting of phenol-degrading bacteria and plant in detoxification of phenol. After the experiments the harvested CPT still exhibited remarkable bioremediation activity. The number of degradative plasmid-carrying endophytic bacteria in the CTP system was just slightly more than in the corn seedlings inoculated with TP13 alone, but the former substantially surpassed the latter in phenol-degrading activity, probably due to stimulation of transfer of the degradative plasmids among endophytic bacteria in plant tissues. More degradative plasmid-carrying bacteria colonized bioremediating soil and plant tissues, and higher plasmid transfer frequency and C23O activity of transconjugant were found in soils for the CTP system compared with other treatments. These results showed that the CTP system is a valuable tool to degrade volatile organic pollutants and transfer of degradative plasmids in plant tissues is important for construction of a mobile plant-endophyte system applied in bioremediation of volatile pollutants.

Keywords: Plant–microbe remediation; Plasmid transfer; Pseudomonas fluorescens P13; Volatile pollutants.