doi: 10.1016/0167-4781(88)90112-1.
Partial purification and characterization of a cellular protein that binds to the SV40 core origin of DNA replication
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- PMID: 2850016
- DOI: 10.1016/0167-4781(88)90112-1
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Partial purification and characterization of a cellular protein that binds to the SV40 core origin of DNA replication
Biochim Biophys Acta.
.
Abstract
A monkey cell factor that interacts specifically with double- and single-stranded DNA sequences in the early domain of the simian virus 40 (SV40) core origin of replication was identified using gel-retention assays. The protein was enriched over 1200-fold using ion-exchange and affinity chromatography on single-strand DNA cellulose. Binding of protein to mutant origin DNA restriction fragments was correlated with replication activity of the mutant DNAs. Exonuclease footprint experiments on single-stranded DNA revealed prominent pause sites in the early domain of the core origin. The results suggest that this cellular protein may be involved in SV40 DNA replication.
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