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. 2016 Dec;5(4):244-251.
doi: 10.1007/s13671-016-0156-3. Epub 2016 Oct 3.

Human as the Ultimate Wound Healing Model: Strategies for Studies Investigating the Dermal Lipidome

Dayanjan S Wijesinghe  1 , Urszula Osinska Warncke  2 , Robert F Diegelmann  3
Affiliations

Human as the Ultimate Wound Healing Model: Strategies for Studies Investigating the Dermal Lipidome

Dayanjan S Wijesinghe et al. Curr Dermatol Rep. 2016 Dec.

Abstract

Purpose of review: Educate the reader of the multiple roles undertaken by the human epidermal lipidome and the experimental techniques of measuring them.

Recent findings: Damage to skin elicits a wound healing process that is capped by the recreation of the lipid barrier. In addition to barrier function, lipids also undertake an active signaling role during wound healing. Achievement of these multiple functions necessitates a significant complexity and diversity in the lipidome resulting in a composition that is unique to the human skin. As such, any attempts to delineate the function of the lipidome during the wound healing process in humans need to be addressed via studies undertaken in humans.

Summary: The human cutaneous lipidome is unique and play a functionally significant role in maintaining barrier and regulating wound healing. Modern mass spectrometry and Raman spectroscopy based methods enable the investigation epidermal lipidome with respect to those functions.

Keywords: analytical methods; bioactive lipids; human; sampling techniques; wound healing.

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Conflict of interest statement

Conflict of Interest Dayanjan S Wijesinghe, Urszula Osinska Warncke, and Robert F. Diegelmann declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1. Alteration in the NS (Cer 2) ceramide profile during wound healing in humans
Lipids were extracted from a 1cm portion of PTFE implants inserted into the upper arm of healthy volunteers and removed on days 3, 5, 7 and 14. A 100 mm skin punch biopsy was used as the baseline (day 0). Lipids were extracted from those samples and subjected to targeted analysis via LC tandem mass spectrometry. The data shown is the average lipid content from 7 volunteers (n=7) normalized to total sphingolipids ± SD. The lipid content is depicted in pmol specific lipid/pmol total sphingolipids found in 1cm of PTFE insert.
See this image and copyright information in PMC

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