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. 2017 May 9:5:e3330.
doi: 10.7717/peerj.3330. eCollection 2017.

Divergent effect of fluoxetine on the response to physical or chemical stressors in zebrafish

Murilo S Abreu #  1 , Ana Cristina V V Giacomini #  1   2 , Gessi Koakoski #  1 , Angelo L S Piato #  3 , Leonardo J G Barcellos #  1   4
Affiliations

Divergent effect of fluoxetine on the response to physical or chemical stressors in zebrafish

Murilo S Abreu et al. PeerJ. .

Abstract

Fluoxetine is a selective serotonin reuptake inhibitor that increases serotonin concentration in the central nervous system and modulates various systems, including the control of sympathetic outflow and the hypothalamus-pituitary-adrenal. However, it is not yet established whether fluoxetine can modulate the responses to stressors stimulants (physical or chemical) that trigger cortisol response in zebrafish. We demonstrate that fluoxetine blunts the response to physical stress, but not to chemical stress.

Keywords: Alarm substance; Blood; Chemical stressors; Cortisol; Physical stressors; SSRI; Serotonin; Stress response.

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Conflict of interest statement

Angelo L.S. Piato is an Academic Editor for PeerJ.

Figures

Figure 1
Figure 1. Schematic representation of the experimental design.
Figure 2
Figure 2. Effects of physical acute stressors (spatial restriction or chasing) on cortisol levels in whole-body zebrafish.
Data were expressed as mean + SEM. Two-way ANOVA followed by Dunnett’s post hoc test. FLU (fluoxetine). *p < 0.05 and ****p < 0.0001.
Figure 3
Figure 3. Effects of chemical acute stressors (alarm substance or blood) on cortisol levels in whole-body zebrafish.
Data were expressed as mean + SEM. Two-way ANOVA followed by Dunnett’s post hoc test. FLU (fluoxetine). *p < 0.05.
See this image and copyright information in PMC

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