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. 1988 Nov;54(11):2877-80.
doi: 10.1128/aem.54.11.2877-2880.1988.

Delivery system for creation of one-step in vivo lac gene fusions in Pseudomonas spp. involved in biological control

D J O'Sullivan  1 , F O'Gara
Affiliations

Delivery system for creation of one-step in vivo lac gene fusions in Pseudomonas spp. involved in biological control

D J O'Sullivan et al. Appl Environ Microbiol. 1988 Nov.

Abstract

The suicide plasmid pVA838 carrying the operon fusion transposon Tn5-lac was used as a delivery system to introduce Tn5-lac into Pseudomonas sp. strain M114. Random, in vivo lac gene fusions were successfully isolated in a one-step conjugation approach with this vector system. Tn5-lac-containing exconjugants were recovered at a frequency of approximately 10(-7) per recipient. However, when the mating temperature was increased from the normal growth temperature (28 degrees C) to 34 degrees C, the frequency was increased to approximately 10(-4) per recipient. A number of in vivo lac gene fusions were isolated and characterized in strain M114, a potentially important bacterium for biological control purposes.

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References

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