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. 2017 May 16:41:4A.11.1-4A.11.13.
doi: 10.1002/cpsc.25.

Rhesus Macaque iPSC Generation and Maintenance

Ravi Chandra Yada  1 , So Gun Hong  1 , Yongshun Lin  2 , Thomas Winkler  1 , Cynthia E Dunbar  1
Affiliations

Rhesus Macaque iPSC Generation and Maintenance

Ravi Chandra Yada et al. Curr Protoc Stem Cell Biol. .

Abstract

The rhesus macaque (Macaca mulatta) is physiologically and phylogenetically similar to humans, and therefore represents an invaluable model for the pre-clinical assessment of the safety and feasibility of iPSC-derived cell therapies. The use of an excisable polycistronic lentiviral STEMCCA vector to reprogram rhesus fibroblasts or bone marrow stromal cells (BMSCs) into RhiPSCs is described. After reprogramming, the pluripotency transgenes can be removed by transient expression of Cre, leaving a residual genetic tag that may be useful for identification of RhiPSC-derived tissues in vivo. Finally, the steps to maintain pluripotency during passaging of RhiPSCs, required for successful utilization of RhiPSCs, is described. © 2017 by John Wiley & Sons, Inc.

Keywords: induced pluripotent stem cells (iPSCs); non-human primate; reprogramming; rhesus macaque.

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Figures

Figure 1
Figure 1
Identifying good quality RhiPSCs for passaging. (A) RhiPSC colony with ESC-like morphology that is ideal for passaging. (B) A representative RhiPSC colony of poor quality with obvious differentiation and cell death. Images were taken at 40×.
See this image and copyright information in PMC

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