Association of PECAM-1 Gene Polymorphisms with Kawasaki Disease in Chinese Children

Abstract

Kawasaki disease (KD) is an acute systemic vasculitis complicated by development of coronary artery lesions. PECAM-1 is a kind of cell adhesion molecule, which plays an important role in coronary artery disease. The relationship between PECAM-1 gene polymorphisms and their susceptibility to Kawasaki diseases (KD) is still unclear. In our study, we examined the PECAM-1 gene polymorphisms in 44 KD patients and 59 healthy children and revealed the correlation of PECAM-1 gene polymorphisms in KD children with and without coronary artery lesions (CAL).

Figure 1

Representative results of the two PECAM-1 polymorphism sites in two groups. Lane M, DNA size marker. Lane 1, KD without CAL; Lane 2, KD with CAL; Lane 3, control's sample. (a) PECAM-1 +373 C/G gene. (b) PECAM-1 +1688 A/G gene.

Figure 2

PCR-RFLP assay for PECAM-1 gene (+1688 A/G) and (+373 C/G) polymorphism. Representative electrophoresis of PCR products cleaved with restriction endonuclease Pvu II and Nhe I was shown. Lane M: DNA molecular weight marker. (a) PECAM-1 (+373 C/G) gene. Lane 1: 373CC homozygous genotype, 188 bp; Lane 2: 373CG heterozygous genotype, 255 bp and 188 bp; Lane 3: 373GG homozygous genotype, 255 bp. The 37 bp in 373 CC and 373 CG genotypes was not visible in electrophoresis gel due to its too small size. (b) PECAM-1 (+1688 A/G) gene. Lane 1: 1688AG heterozygous genotype, 338 bp, 219 bp, and 119 bp; Lane 2: 168AA homozygous genotype, 338 bp; and Lane 3: 1688GG homozygous genotype, 219 bp and 119 bp. PECAM-1: platelet endothelial cell adhesion molecule-1.