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. 1988 Oct;174(1):32-7.
doi: 10.1016/0003-2697(88)90515-5.

A continuous spectrophotometric assay for the enzymatic marker glucose 6-phosphatase

M Stio  1 , P Vanni, G Pinzauti
Affiliations

A continuous spectrophotometric assay for the enzymatic marker glucose 6-phosphatase

M Stio et al. Anal Biochem. 1988 Oct.

Abstract

A continuous spectrophotometric assay for glucose 6-phosphatase is described. The method uses glucose dehydrogenase and mutarotase as ancillary enzymes. Glucose 6-phosphatase activity is measured by following NADH formation at 340 nm. The method is linear, at least up to 38 mU in the test which corresponds to a delta E of 0.24 min-1, when the enzyme is assayed in a microsomal fraction. We also discuss the method's suitability for subcellular fractionation. No other continuous assay for this important enzymatic marker of the endoplasmic reticulum is currently available.

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