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. 2017 Apr;12(2):99-106.
doi: 10.4103/1735-5362.202448.

Biochemical and histopathological effects of green tea nanoparticles in ironized mouse model

Hidayat Sulistyo  1 , Dhadhang Wahyu Kurniawan  2 , Lantip Rujito  3
Affiliations

Biochemical and histopathological effects of green tea nanoparticles in ironized mouse model

Hidayat Sulistyo et al. Res Pharm Sci. 2017 Apr.

Abstract

Transfusion in the treatment of thalassemia gives rise to iron deposits in many organs. Since there are many obstacles in the use of deferoxamin (DFO) as an iron chelating agent, it is important to find another alternative therapy that can act as iron chelation. The study aims to compare the histopathological pictures of the heart and spleen in iron-induced rats after administration of DFO and nanoparticles of green tea extract. The research used experimental research design with a post-test only control group. Experimental nano green teas were divided into four treatment groups; no diet, DFO supplementation, nano green tea supplementation, and a combination of both DFO and green tea. Ferritin and glutathione peroxides were used as biochemical parameters, and histopathological pictures of the heart and spleen were recorded. The study showed that there was significant improvement in the rats receiving DFO and nanoparticles of green tea compared with the rats in the no diet group. The study also reported that nano green tea has an effect comparable to DFO.

Keywords: Green tea; Iron chelator; Nanoparticle; Thalassemia; Transfusion.

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Figures

Fig. 1
Fig. 1
Size distribution of nanoparticles green tea extract using particle size analyzer (PSA).
Fig. 2
Fig. 2
Nanoparticles of green tea extract images using transmission electron microscopy (TEM).
Figure 3
Figure 3
The value of zeta potential of green tea extract nanoparticles.
Fig. 4
Fig. 4
The difference of ferritin level shortly after the induction period of ferrous sulfate (dark color) and after administration of DFO and green tea nanoparticles (light color). (1) control no diet; (2) DFO diet; (3) nano green tea diet; and (4) DFO and nano green tea diet.
Fig. 5
Fig. 5
The different level of the glutathione peroxidase (GPx) enzyme shortly after the induction period of ferrous sulfate (dark color) and after administration of DFO and green tea nanoparticles (light color). (1) group with no diet; (2) DFO diet; (3) nano green tea diet; and (4) DFO and nano green tea diet.
Fig. 6
Fig. 6
Histopathological features in myocardium hematoxylin and eosin staining. There is no hemosiderosis, only some cells undergo necrosis. (A) Control no diet; (B) DFO diet; (C) nano green tea diet, and (D) DFO and nano green tea diet.
Fig. 7
Fig. 7
Hemosiderin laden of macrophages in the spleen of mice induced ferrous sulfate. (A) control no diet; (B) DFO diet; (C) nano green tea diet; and (D) DFO and nano green tea diet.
See this image and copyright information in PMC

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