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. 1988 Nov;8(6):459-63.
doi: 10.1007/BF00916951.

Probing the human B-cell repertoire: isolation of Epstein-Barr virus (EBV)-transformed human B lymphocytes making antibodies with a common idiotope that have different antigen-binding specificities

Y Uchigata  1 , B S Prabhakar, A L Notkins
Affiliations

Probing the human B-cell repertoire: isolation of Epstein-Barr virus (EBV)-transformed human B lymphocytes making antibodies with a common idiotope that have different antigen-binding specificities

Y Uchigata et al. J Clin Immunol. 1988 Nov.

Abstract

Human peripheral B lymphocytes were transformed with Epstein-Barr virus and probed with an antiidiotypic antibody made against a human monoclonal autoantibody. Six cell lines were isolated that produced antibodies bearing a common idiotope. Despite the presence of this common idiotope, the antibodies showed antigen-binding specificities different from that of the parental antibody to which the antiidiotypic antibody was made. By probing Epstein-Barr virus-transformed cells with well-characterized antiidiotypic antibodies, it should now be possible to isolate and determine the frequency of B cells bearing specific idiotopes in the human repertoire and to study the antigen-binding properties of these antibodies.

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