Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Sep;8(9):3740-7.
doi: 10.1128/mcb.8.9.3740-3747.1988.

Characterization of mutant p53-hsp72/73 protein-protein complexes by transient expression in monkey COS cells

H W Stürzbecher  1 , C Addison, J R Jenkins
Affiliations

Characterization of mutant p53-hsp72/73 protein-protein complexes by transient expression in monkey COS cells

H W Stürzbecher et al. Mol Cell Biol. 1988 Sep.

Abstract

Several mutant, but not wild-type, p53 proteins form complexes with hsp72/73 heat shock-related proteins in simian virus 40-transformed monkey COS cells. We carried out a detailed biochemical and structural mapping analysis of p53 and report here that p53-hsp72/73 complex formation showed considerable structural specificity. Such complexes were remarkably stable, but unlike analogous complexes formed between p53 and simian virus 40 T antigen, they did not form in in vitro association assays. p53-hsp72/73 complex formation in vivo appears to be dependent on aspects of mutant p53 protein conformation. However, absence of the conformation-sensitive epitope recognized by monoclonal antibody PAb 246 was not reliably diagnostic of such complexes, nor was p53-hsp72173 binding reliably diagnostic of oncogenic activation.

PubMed Disclaimer

References

    1. EMBO J. 1985 Mar;4(3):699-706 - PubMed
    1. Nature. 1984 Dec 13-19;312(5995):651-4 - PubMed
    1. EMBO J. 1985 Dec 16;4(13A):3385-91 - PubMed
    1. Nature. 1986 Mar 13-19;320(6058):182-4 - PubMed
    1. Cell. 1986 Apr 11;45(1):3-13 - PubMed