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. 2017 Jul 18;8(29):47175-47183.
doi: 10.18632/oncotarget.17565.

Aquaporins 1, 3 and 8 expression in irritable bowel syndrome rats' colon via NF-κB pathway

Guanqun Chao  1 , Shuo Zhang  2
Affiliations

Aquaporins 1, 3 and 8 expression in irritable bowel syndrome rats' colon via NF-κB pathway

Guanqun Chao et al. Oncotarget. .

Abstract

Objective: Our research was to detect the expression of aquaporins. NF-κB in Irritable bowel syndrome (IBS) rat models' colon so as to find novel pathogenesisof IBS.

Results: The expression of AQP1, AQP3, and AQP8 of IBS model group was down-regulated while NF-κB p65 was up-regulated comparing with control group (p < 0.05), and the expression of AQP1, AQP3, and AQP8 of inhibitor group was up-regulated while NF-κB p65 was down-regulated comparing with IBS model group (p < 0.05).

Materials and methods: 18 adult female SD big rats were divided into three groups:the rats in control group were normal rats,the rats in IBS model group and the rats of inhibitor group were injected with the inhibitor of NF-κB (PDTC). Immunohistochemical technique and western blot were performed to detect the expression of AQP1, AQP3, AQP8 and NF-κB p65. RT-PCR was performed to detect the expression of AQP1, AQP3, and AQP8.

Conclusions: Liquid water metabolic abnormalities and intestine permeability alteration might be the mechanism of IBS by down-regulating AQP1, AQP3 and AQP8 via NF-κB pathway.

Keywords: NF-κB; aquaporins; irritable bowel syndrome.

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Conflict of interest statement

CONFLICTS OF INTEREST

None.

Figures

Figure 1
Figure 1. The expression of AQP1 in the colon by immunohistochemical technique (*400) AQP1 positive reaction material presents brown, the cell membrane and cytoplasm were dyed
Figure 2
Figure 2. The expression of AQP3 in the colon by immunohistochemical technique (*400) AQP3 positive reaction material presents brown, the cell membrane and cytoplasm were dyed
Figure 3
Figure 3. The expression of AQP8 in the colon by immunohistochemical technique (*400) AQP8 positive reaction material presents brown, the cell membrane and cytoplasm were dyed
Figure 4
Figure 4. The expression of NF-κB p65 in the colon by immunohistochemical technique (*400) NF-kB p65 positive reaction material presents brown, the cell nucleus was dyed, the cell membrane and cytoplasm were not dyed
Figure 5
Figure 5. The expressions of AQP1, AQP3, AQP8 and NF-κB p65 in the colon by Western blot technique
The expressions of AQP1, AQP3 and AQP8 protein of model group were less than the control group (p < 0.05), and the expressions of inhibitor group were more than model group (p < 0.05), The expressions of NF-κB p65 protein of model group were more than the control group (p < 0.05), and the expressions of inhibitor group were less than model group (p < 0.05).
See this image and copyright information in PMC

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