Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2017 Aug:254:340-346.
doi: 10.1016/j.psychres.2017.04.034. Epub 2017 Apr 22.

Measuring BDNF in saliva using commercial ELISA: Results from a small pilot study

Charlotte Vrijen  1 , Hendrika M Schenk  2 , Catharina A Hartman  3 , Albertine J Oldehinkel  4
Affiliations
Free article
Comparative Study

Measuring BDNF in saliva using commercial ELISA: Results from a small pilot study

Charlotte Vrijen et al. Psychiatry Res. 2017 Aug.
Free article

Abstract

Brain-derived neurotrophic factor (BDNF) is a protein often studied in psychiatric populations. Commercial ELISA kits have been validated for measuring BDNF in blood plasma and serum, but blood collection is an invasive method which cannot always be used. The aim of this pilot study was to explore the noninvasive alternative of measuring BDNF in saliva. Three different commercial ELISA kits were used to analyze parallel plasma and saliva samples from six healthy adults. In total 33 plasma and 33 saliva samples were analyzed according to manufacturers' standard protocols. BDNF was successfully measured in plasma in two of the three kits, of which the results correlated highly (rs =.88). BDNF could not be measured reliably in saliva. The results of this pilot study suggest that techniques of commercial BDNF kits may not be ready for noninvasive saliva measurements, which limits the sampling frequency and settings.

Keywords: BDNF; ELISA; Plasma; Saliva.

PubMed Disclaimer

Publication types

Substances

LinkOut - more resources