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. 2017 Sep;137(9):2030-2033.
doi: 10.1016/j.jid.2017.05.003. Epub 2017 May 18.

Staphylococcus aureus Lipoteichoic Acid Inhibits Keratinocyte Differentiation through a p63-Mediated Pathway

Anne M Brauweiler  1 , Clifton F Hall  1 , Elena Goleva  1 , Donald Y M Leung  2
Affiliations

Staphylococcus aureus Lipoteichoic Acid Inhibits Keratinocyte Differentiation through a p63-Mediated Pathway

Anne M Brauweiler et al. J Invest Dermatol. 2017 Sep.
No abstract available

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Conflict of interest statement

CONFLICT OF INTEREST

The authors state no conflict of interest.

Figures

Fig. 1
Fig. 1. Staphylococcus aureus LTA induces global changes in transcription that inhibit expression of genes involved in epidermal differentiation
(a) Primary human keratinocytes were treated with media alone or 10 ug/ml LTA for 24 hours. Isolated RNA was subjected to microarray analysis. Multiple biological processes were significantly affected by treatment with staphylococcal LTA. (b) Microarray analysis shows that genes involved in keratinocyte differentiation were down regulated by LTA.
Fig 2
Fig 2. LTA converts p63 into an inhibitor of keratinocyte differentiation
(a) Keratinocytes transfected with control or p63 siRNA for 24 hours were either trypsinized and re-plated, or allowed to remain adherent. Photomicrographs show that p63 deficient keratinocytes remain adherent and viable in the absence trypsinization. Bar, 10µm (b) Keratinocytes were transfected with control or p63 siRNA for 24 hours. Cells were then induced to differentiate with calcium in the presence or absence of LTA. RT-PCR analysis of the indicated genes was performed on cells treated for one day (Cyclin A1), or four days (KRT-1, KRT-5, KRT-10, DSC-1, and DSG-1). Genes induced upon early keratinocyte differentiation were prominently down-regulated by LTA. Notably, the inhibition of differentiation induced by LTA is absent in p63 siRNA transfected cells. (c) Cells transfected with control or p63 siRNA were differentiated with calcium in the presence and absence of LTA. Following a four day incubation, cells were analyzed by Western blot for expression of the indicated proteins. Data are mean ± SEM, n = 3. **P<0.01; ***P<0.001 (LTA treated control vs. LTA treated p63 siRNA keratinocytes).
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