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. 2017 May;13(5):3586-3598.
doi: 10.3892/ol.2017.5909. Epub 2017 Mar 24.

Evaluation of microRNA-205 expression as a potential triage marker for patients with low-grade squamous intraepithelial lesions

Hong Xie  1   2 , Ingrid Norman  3 , Anders Hjerpe  4 , Tomislav Vladic  5 , Catharina Larsson  2 , Weng-Onn Lui  2 , Ellinor Östensson  5   6 , Sonia Andersson  5
Affiliations

Evaluation of microRNA-205 expression as a potential triage marker for patients with low-grade squamous intraepithelial lesions

Hong Xie et al. Oncol Lett. 2017 May.

Abstract

High-risk human papillomavirus (HPV) testing is a recommended triage approach for females with atypical squamous cells of undetermined significance (ASCUS), but due to its poor specificity this approach is not recommended for patients with low-grade squamous intraepithelial lesions (LSIL). The objective of the current study was to determine microRNA (miR)-205 expression levels in liquid-based cytology (LBC) samples, and evaluate their ability to predict cervical intraepithelial neoplasia grade 2/3 or worse (CIN2/3+) in females with minor cytological abnormalities. LBC samples were obtained from patients attending the Swedish Cervical Cancer Screening Program. The Mann-Whitney U test, one-way analysis of variance, Kruskal-Wallis test, Spearman rank order correlation analysis, and Pearson's χ2 test were used to assess the results. Accuracy analyses indicated that high miR-205 expression had a significantly higher specificity to high-risk HPV testing, and a sensitivity similar to that of high-risk HPV testing to predict CIN2+ and CIN3+ in women with LSIL, but not those with high-grade squamous intraepithelial lesions. Although further research is required for females with LSIL, miR-205 expression in LBC samples may be a novel triage marker for, or a beneficial supplement to high-risk-HPV testing in these patients.

Keywords: cervical intraepithelial lesions; human papillomavirus; liquid-based cytology; microRNA-205; specificity.

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