Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 May;6(5):539-544.
doi: 10.3892/br.2017.888. Epub 2017 Apr 10.

Rosehip inhibits xanthine oxidase activity and reduces serum urate levels in a mouse model of hyperuricemia

Hidetomo Kikuchi  1 , Satomi Kogure  1 , Rie Arai  1 , Kouki Saino  1 , Atsuko Ohkubo  2 , Tadashi Tsuda  1 , Katsuyoshi Sunaga  1
Affiliations

Rosehip inhibits xanthine oxidase activity and reduces serum urate levels in a mouse model of hyperuricemia

Hidetomo Kikuchi et al. Biomed Rep. 2017 May.

Abstract

Rosehip, the fruit of Rosa canina L., has traditionally been used to treat urate metabolism disorders; however, its effects on such disorders have not been characterized in detail. Therefore, the present study investigated the effects of hot water, ethanol and ethyl acetate extracts of rosehip on xanthine oxidase (XO) activity in vitro. In addition, the serum urate lowering effects of the rosehip hot water extract in a mouse model of hyperuricemia (male ddY mice, which were intraperitoneally injected with potassium oxonate) were investigated. Furthermore, the influence of rosehip hot water extract on CYP3A4 activity, which is the most important drug-metabolizing enzyme from a herb-drug interaction perspective, was investigated. Rosehip extracts of hot water, ethanol and ethyl acetate inhibited XO activity [half maximal inhibitory concentration (IC50) values: 259.6±50.6, 242.5±46.2 and 1,462.8±544.2 µg/ml, respectively]. Furthermore, the administration of 1X rosehip hot water extract significantly reduced the levels of serum urate at 8 h, which was similar when compared with the administration of 1 mg/kg allopurinol. Rosehip hot water extract only marginally affected CYP3A4 activity (IC50 value, >1 mg/ml). These findings indicate that rosehip hot water extract may present as a functional food for individuals with a high urate level, and as a therapeutic reagent for hyperuricemic patients.

Keywords: CYP3A4 activity; functional foods; hyperuricemic mice; rosehip extract; self-medication; xanthine oxidase activity.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Diagram of the timeline of treatment with rosehip hot water extracts and allopurinol in the hyperuricemia model mice.
Figure 2.
Figure 2.
Effect of rosehip extracts on XO activity. (A) Hot water extract, (B) ethanol extract, and (C) ethyl acetate extract of rosehip dose-dependently reduced XO activity. (D) The IC50 values of each extract were calculated and compared. Data are reported as means ± standard deviation. *P<0.05 vs. ethyl acetate extraction. XO, xanthine oxidase; IC50, half maximal inhibitory concentration.
Figure 3.
Figure 3.
Levels of serum urate in hyperuricemic mice treated with vehicle, 1 mg/ml allopurinol, 5 mg/ml allopurinol, 0.5X rosehip extract, and 1X rosehip extract. Data are presented as means ± standard error of the mean. *P<0.05 and **P<0.01 vs. control group; †P<0.05 and ‡P<0.01 vs. each case at time 0.
Figure 4.
Figure 4.
Effect of rosehip extracts on CYP3A4 activity. Data are presented as means ± standard deviation.
See this image and copyright information in PMC

References

    1. Galland L. Functional Foods: Health Effects and Clinical Applications. Elsevier Ltd.; 2013. pp. 366–371.
    1. World Health Organization (WHO): The role of the pharmacist in self-care and self-medication. WHO; Geneva: 1998. p. 15.
    1. Kikuchi H, Yuan B, Yuhara E, Imai M, Furutani R, Fukushima S, Hazama S, Hirobe C, Ohyama K, Takagi N, et al. Involvement of histone H3 phosphorylation via the activation of p38 MAPK pathway and intracellular redox status in cytotoxicity of HL-60 cells induced by Vitex agnus-castus fruit extract. Int J Oncol. 2014;45:843–852. - PubMed
    1. Kikuchi H, Yuan B, Yuhara E, Takagi N, Toyoda H. Involvement of histone H3 phosphorylation through p38 MAPK pathway activation in casticin-induced cytocidal effects against the human promyelocytic cell line HL-60. Int J Oncol. 2013;43:2046–2056. - PubMed
    1. Kikuchi H, Yuan B, Nishimura Y, Imai M, Furutani R, Kamoi S, Seno M, Fukushima S, Hazama S, Hirobe C, et al. Cytotoxicity of Vitex agnus-castus fruit extract and its major component, casticin, correlates with differentiation status in leukemia cell lines. Int J Oncol. 2013;43:1976–1984. - PubMed