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Controlled Clinical Trial
. 2017 Jul-Aug;21(4):391-395.
doi: 10.1016/j.bjid.2017.03.016. Epub 2017 May 20.

Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study

Emine Akçay  1 , Ahmet Çarhan  2 , Gözde Hondur  3 , Zeliha Koçak Tufan  4 , Necati Duru  5 , Selçuk Kılıç  6 , Ezgi Naz Ensari  7 , Nagihan Uğurlu  1 , Nurullah Çağıl  1
Affiliations
Controlled Clinical Trial

Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study

Emine Akçay et al. Braz J Infect Dis. 2017 Jul-Aug.

Abstract

Background: Viral conjunctivitis are the most frequent infections in ophthalmology clinics. The diagnosis is usually relying on clinical findings and medical history. However, topical antibiotics are often used unnecessarily addition to symptomatic treatment because of unsure agents. We aimed to detect the Adenovirus, Coxsackievirus and Enterovirus from conjunctiva and pharyngeal samples of patients.

Methods: The conjunctiva and pharyngeal samples of the patients with conjunctivitis were taken by Virocult transport media and kept at -80̊C up to study day. Adenovirus spp, Enterovirus 70 and Enterovirus 71, Coxsackie A24 and Coxsackie A16 were detected by real-time PCR. Samples from healthy health care workers of ophthalmology clinic were used for control group.

Results: A total of 176 samples (conjunctival and pharyngeal samples of 62 patient and 26 healthy subjects) were included. The mean age of 34 (55.7%) male and 27 (44.3%) female patients was 34±17. Twenty five (40.3%) of the patients were receiving antibiotic drops at first visit. The main etiologic agent in conjunctival samples was found to be Adenovirus (46/62, 74.2%) followed by Enterovirus 70 (4/62, 6.4%) and Enterovirus 71 (4/62, 6.4%). Coxsackievirus 16 and 24 were also found in 2 patients (1/62 each, 1.6%). Pharyngeal samples were also positive for Adenovirus (20/62, 32.3%), Enterovirus 70 and 71 (7/62, 11.3% and 5/62, 8.1% respectively), Coxsackievirus 16 and 24 (2/62, 3.2% and 1/61, 1.6%).

Conclusions: It is very difficult in viral conjunctivitis to make clinical differentiation caused by different agents because of common clinical signs and symptoms. In routine clinical work, the viral conjunctivitis usually related with Adenovirus. But almost one fourth of the patients' conjunctivitis were not related to Adenovirus, which shows the importance of the laboratory diagnostics. True diagnosis plays an important role on prevention of contamination and unnecessary use of antibiotics in viral conjunctivitis.

Keywords: Adenovirus; Conjunctivitis; PCR; Viral.

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References

    1. Uchio E., Takeuchi S., Itoh N., et al. Clinical and epidemiological features of acute follicular conjunctivitis with special reference to that caused by herpes simplex virus type 1. Br J Ophthalmol. 2000;84:968–972. - PMC - PubMed
    1. Woodland R.M., Darougar S., Thaker U., et al. Causes of conjunctivitis and keratoconjunctivitis in Karachi, Pakistan. Trans R Soc Trop Med Hyg. 1992;86:317–320. - PubMed
    1. O’Brien T.P., Jeng B.H., McDonald M., Raizman M.B. Acute conjunctivitis: truth and misconceptions. Curr Med Res Opin. 2009;25:1953–1961. - PubMed
    1. Lynch J.P., Kajon E.A. Adenovirus: epidemiology, global spread of novel serotypes, and advances in treatment and prevention. Semin Respir Crit Care Med. 2016;37:586–602. - PMC - PubMed
    1. Li J., Yang Y., Lin C., et al. Etiology of acute conjunctivitis due to coxsackievirus A24 variant, human adenovirus, herpes simplex virus, and chlamydia in Beijing, China. Jpn J Infect Dis. 2014;67:349–355. - PubMed

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