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Review
. 2017:2017:9831534.
doi: 10.1155/2017/9831534. Epub 2017 Apr 30.

Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications

Anna Gilpin  1 , Yong Yang  1
Affiliations
Review

Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications

Anna Gilpin et al. Biomed Res Int. 2017.

Abstract

As the gap between donors and patients in need of an organ transplant continues to widen, research in regenerative medicine seeks to provide alternative strategies for treatment. One of the most promising techniques for tissue and organ regeneration is decellularization, in which the extracellular matrix (ECM) is isolated from its native cells and genetic material in order to produce a natural scaffold. The ECM, which ideally retains its inherent structural, biochemical, and biomechanical cues, can then be recellularized to produce a functional tissue or organ. While decellularization can be accomplished using chemical and enzymatic, physical, or combinative methods, each strategy has both benefits and drawbacks. The focus of this review is to compare the advantages and disadvantages of these methods in terms of their ability to retain desired ECM characteristics for particular tissues and organs. Additionally, a few applications of constructs engineered using decellularized cell sheets, tissues, and whole organs are discussed.

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Figures

Figure 1
Figure 1
Process of engineering tissues using decellularized ECM.
See this image and copyright information in PMC

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