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. 2017 Oct;143(10):1941-1946.
doi: 10.1007/s00432-017-2444-0. Epub 2017 May 24.

Analysis of primary-miRNA-3662 and its mature form may improve detection of the lung adenocarcinoma

Tomasz Powrózek  1 , Radosław Mlak  2 , Marcin Dziedzic  3 , Teresa Małecka-Massalska  2 , Dariusz Sagan  4
Affiliations

Analysis of primary-miRNA-3662 and its mature form may improve detection of the lung adenocarcinoma

Tomasz Powrózek et al. J Cancer Res Clin Oncol. 2017 Oct.

Abstract

Introduction: Because of the alarming data concerning lung cancer morbidity and mortality, investigation of new molecular markers allowing early cancer detection is desirable. In the present study, we investigated the potential role of miRNA-3662 precursor (pri-miRNA-3662) as potential novel diagnostic marker of lung adenocarcinoma (AC).

Materials and methods: Expression of miRNA-3662 and pri-miRNA-3662 was analyzed in 56 fresh-frozen tissues of non-small cell lung cancer and corresponding adjacent non-cancerous tissues using (NCT) qRT-PCR. Using receiver operating curves (ROC) analysis, the diagnostic accuracy of both studied markers for AC detection was assessed.

Results: miRNA-3662 and its precursor were significantly overexpressed in AC compared to squamous-cell carcinoma (SCC) and NCT. Combined analysis of pri-miRNA-3662 and mature miRNA-3662 allowed to distinguish AC tissue from SCC with sensitivity of 96% and specificity of 85.7% (AUC = 0.963), and SCC from non-cancerous tissue with 92% sensitivity and 92% specificity (AUC = 0.979).

Conclusions: miRNA-3662 and its precursor are potentially involved in AC development. pri-miRNA seem to be novel interesting group of potential cancer biomarkers, because they demonstrate high diagnostic accuracy for tumor detection.

Keywords: Adenocarcinoma; Lung cancer; miRNA; pri-miRNA.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1
Differences in expression of pri-miRNA-3662 and miRNA-3662 in adenocarcinoma (AC), squamous-cell carcinoma (SCC) and non-cancerous (NT) tissues
Fig. 2
Fig. 2
a Relative expression level of pri-miRNA-3662 in all AC samples and in corresponding adjacent non-cancerous tissues (NCT); b differences in pri-miRNA-3662 expression between AC and NCT: box and whisker represent median (band inside the box) and interquartile range (1st and 3rd quartile), whereas ends of the whiskers present minimal and maximal expression of studied samples; c relative expression level of miRNA-3662 in all AC samples and in corresponding NCT samples; d differences in miRNA-3662 expression between AC and NCT: box and whisker interpreted as in b
Fig. 3
Fig. 3
Diagnostic accuracy of studied AC markers conducted by ROC analysis with AUC calculation: a combined analysis of pri-miRNA-3662 and miRNA-3662 and their diagnostic accuracy for distinguishing AC from SCC; b combined analysis of pri-miRNA-3662 and miRNA-3662 and their diagnostic accuracy for distinguishing ACC from NCT
See this image and copyright information in PMC

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