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. 2018:1697:153-171.
doi: 10.1007/7651_2017_43.

Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation

Guanghu Wang  1 , Stefka D Spassieva  2 , Erhard Bieberich  3   4
Affiliations

Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation

Guanghu Wang et al. Methods Mol Biol. 2018.

Abstract

Bioactive sphingolipids are important regulators for stem cell survival and differentiation. Most recently, we have coined the term "morphogenetic lipids" for sphingolipids that regulate stem cells during embryonic and postnatal development. The sphingolipid ceramide and its derivative, sphingosine-1-phosphate (S1P), can act synergistically as well as antagonistically on embryonic stem (ES) cell differentiation. We show here simple as well as state-of-the-art methods to analyze sphingolipids in differentiating ES cells and discuss new protocols to use ceramide and S1P analogs for the guided differentiation of mouse ES cells toward neuronal and glial lineage.

Keywords: Apoptosis; Ceramide; Neuroprogenitor; Oligodendrocyte precursor; Sphingolipid; Sphingosine-1-phosphate; Teratoma.

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Figures

Figure 1
Figure 1
Structure of the sphingolipids and sphingolipid analogs used for stem cell differentiation
Figure 2
Figure 2
Different stages of mouse ES cell differentiation
Figure 3
Figure 3
Assembly for TLC
See this image and copyright information in PMC

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