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Comparative Study
. 2017 Jul;58(4):786-792.
doi: 10.3349/ymj.2017.58.4.786.

Comparison of the ImmunoCAP Assay and AdvanSure™ AlloScreen Advanced Multiplex Specific IgE Detection Assay

Kyung Hee Park  1   2 , Jongsun Lee  2 , Sang Chul Lee  1 , Young Woong Son  1 , Da Woon Sim  1   2 , Jae Hyun Lee  1   2 , Jung Won Park  1   3
Affiliations
Comparative Study

Comparison of the ImmunoCAP Assay and AdvanSure™ AlloScreen Advanced Multiplex Specific IgE Detection Assay

Kyung Hee Park et al. Yonsei Med J. 2017 Jul.

Abstract

Purpose: The AdvanSure™ AlloScreen assay is an advanced multiplex test that allows for simultaneous detection of specific IgE (sIgE) against multiple allergens. For precise identification of causative allergens in allergic patients, we compared this new multiplex sIgE assay with the ImmunoCAP assay, which is currently the gold-standard method for sIgE detection.

Materials and methods: Serum samples from 218 Korean allergic disease patients were used to compare the ImmunoCAP and AlloScreen assays with respect to the following 13 allergens: Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat and dog dander, Alternaria, birch, oak, ragweed, mugwort, rye grass, and food allergens (egg white, cow's milk, peanuts).

Results: A total of 957 paired tests using the 13 allergens were compared. The total agreement ratio ranged from 0.74 (oak) to 0.97 (Alternaria). With respect to class association analyses, the gamma index ranged from 0.819 (rye grass) to 0.990 (Alternaria). The intra-class correlation coefficients for house dust mites, cat and dog dander, Alternaria, birch, ragweed, egg white, cow's milk, and peanut sIgE titers were >0.8.

Conclusion: The AlloScreen and ImmunoCAP assays exhibited similar diagnostic performance. However, due to methodological differences between the two systems, careful interpretation of their results is needed in clinical applications.

Keywords: Allergens; immunoassay; immunoglobulin E.

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Conflict of interest statement

The authors have no financial conflicts of interest.

Figures

Fig. 1
Fig. 1. Specific IgE titers of each allergen detected by ImmunoCAP and AlloScreen. D. pteronyssinus, Dermatophagoides pteronyssinus; D. farinae, Dermatophagoides farinae.
Fig. 2
Fig. 2. Specific IgE titers of serially diluted serum specimens. Specific IgE titers of Dermatophagoides farinae (D. farina) detected by ImmunoCAP (A) and AlloScreen (B). Specific IgE titers of birch detected by ImmunoCAP (C) and AlloScreen (D). Specific IgE titers of dog detected by ImmunoCAP (E) and AlloScreen (F).
See this image and copyright information in PMC

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