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. 2017 Apr 1;18(4):889-893.
doi: 10.22034/APJCP.2017.18.4.889.

Frequency of Human Papillomavirus (HPV) 16 and 18 Detection in Paraffin- Embedded Laryngeal Carcinoma Tissue

Seyed Zinab Hosseini  1 , Manoochehr Makvandi, Alireza Samarbafzade, Ali Timori, Nastaran Ranjbar, Nader Saki, Nilofar Nisi, Toran Shahani, Mehran Varnaseri, Kambiz Angali Ahmadi
Affiliations

Frequency of Human Papillomavirus (HPV) 16 and 18 Detection in Paraffin- Embedded Laryngeal Carcinoma Tissue

Seyed Zinab Hosseini et al. Asian Pac J Cancer Prev. .

Abstract

Background and Objective: Human papilloma virus (HPV) 16 and HPV18 have been detected in head and neck squamous cell carcinomas (HNSCC) and there is evidence that detection of HPVs would have better prognostic value than patients with HNSCC negative for HPVs. Thus, this study was conducted to evaluate frequency of HPV 16 and HPV 18 genotypes in patients with laryngeal carcinoma. Materials and methods: Fifty formalin-fixed, paraffin-embedded (FFPE) tissue blocks of laryngeal cancers were collected. Sections were prepared at 5 μm and DNA was extracted from each sample and subjected to the polymerase chain reaction (PCR) to detect HPV-16/18 DNA s. Results: All samples were squamous cell carcinomas (SCCs). Overall 14/50 (28%) were positive for HPVs, 8 (18%) with HPV-16 and 6 (12%) with HPV-18. Additionally, 2 (4%) mixed infections of HPV 16 and 18 genotypes were observed among these cases. Conclusions: Overall, 28% of HNSCC samples proved positive for HPV16 and HPV18 genotypes, two high-risk HPV types. It is important to further assess whether such viral infection, could be a risk factor in HNSCC progression.

Keywords: Laryngeal carcinoma; HPV16; HPV18; PCR.

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Figures

Figure 1
Figure 1
The Amplification of E6 Gene Human Papillomavirus (HPV) 16 by PCR. Lane M: DNA Marker (100bp), Lane1: HPV 16-Positive Control (PC), Lanes 3, 4 and 5 Positive HPV 16 Positive Cases, Lane 6: Negative Control (NC).
Figure 2
Figure 2
Detection of HPV 18 DNA in Patients with Larynx Carcinoma by PCR. Lane 1:M DNA Marker (100bp), Lane2: HPV 18-Positive Control (PC) (Hella Cell), Lanes 3, 4 and 5 Positive HPV 18 Cases, Lane 6: Negative Control (NP)
See this image and copyright information in PMC

References

    1. Aguayo F, Khan N, Koriyama C, et al. Human papillomavirus and Epstein-Barr virus infections in breast cancer from chile. Infect Agent Cancer. 2011;6:7. - PMC - PubMed
    1. Almadori G, Cadoni G, Cattani P, et al. Human papillomavirus infection and epidermal growth factor receptor expression in primary laryngeal squamous cell carcinoma. Clin cancer Res. 2001;7:3988–93. - PubMed
    1. American Cancer Society. Oral cavity and oropharyngeal cancer. 2015. Retrieved from http://www.cancer.org/cancer/oralcavityandoropharyngealcancer/detailedgu... .
    1. Ang KK, Harris J, Wheeler R, et al. Weber R, Rosenthal DI, Nguyen-Tan PF, et al., editors. Human papillomavirus and survival of patients with oropharyngeal cancer. N Engl J Med. 2010;363:24–35. - PMC - PubMed
    1. Blitzer GC, Smith MA, Harris SL, et al. Review of the clinical and biologic aspects of human papillomavirus positive squamous cell carcinomas of the head and neck. Int J Radiat Oncol Biol Phy. 2014;88:761–70. - PMC - PubMed