Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1986 Dec;1(6):573-82.
doi: 10.1016/0882-4010(86)90042-2.

Regulation of viral persistence in human glioblastoma and rhabdomyosarcoma cells infected with coronavirus OC43

Affiliations
Comparative Study

Regulation of viral persistence in human glioblastoma and rhabdomyosarcoma cells infected with coronavirus OC43

A R Collins et al. Microb Pathog. 1986 Dec.

Abstract

Cultures of human rhabdomyosarcoma (RD) and human glioblastoma (U87-MG) were compared for their ability to sustain a persistent infection with coronavirus OC43. Within 28 days, infectious virus and hemagglutinin were being produced at high levels in both types of cells. Temperature sensitive plaque variants were recovered at 31 degrees C. In both cell types, the virus caused increased antigen synthesis and cell death, if the temperature was lowered to 31 degrees C. Infectious virus was lost if cells were treated with antiserum to whole virus or if the temperature was raised to 39.5 degrees C. Probing the cured cells with OC43-specific 32P-cDNA showed that cured cells contained no detectable viral RNA. The relative ease of establishment and cure of these persistent infectious makes them attractive as models to study coronavirus regulatory processes.

PubMed Disclaimer

Similar articles

Cited by

References

    1. McIntosh K, Dees JH, Becker WB, Kapikian AZ, Chanock RM. Vol. 57. 1967. Recovery in tracheal organ cultures with novel viruses from patients with respiratory disease; pp. 933–940. (Proc Natl Acad Sci). - PMC - PubMed
    1. Schmidt OW, Cooney MK, Kenny GE. Plaque assay and improved yield of human coronaviruses in a human rhabdomyosarcoma cell line. J Clin Microbiol. 1979;9:722–728. - PMC - PubMed
    1. Pearson J, Mims CA. Selective vulnerability of neural cells and age-related susceptibility to OC43 virus in mice. Arch Virol. 1983;77:109–118. - PMC - PubMed
    1. Pearson J, Mims CA. Differential susceptibility of cultured neural cells to human coronavirus OC43. J Virol. 1985;53:1016–1019. - PMC - PubMed
    1. Burks JS, DeVald BL, Jankovsky LD, Gerdes JC. Two coronaviruses isolated from central nervous system tissue of two multiple sclerosis patients. Science. 1980;209:933–934. - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources