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. 2017:2017:4540124.
doi: 10.1155/2017/4540124. Epub 2017 May 4.

Study of Guanxinning Injection on Regulatory Mechanism of Bcl-2 and Bax by Liquid Nitrogen Freezing-Mediated Femoral Head Necrosis

Xiaofeng Zhang  1 , Xilin Xu  2 , Gerhard Litscher  1   3 , Zemin Sheng  1   4 , Lu Wang  1   3 , Daniela Litscher  3 , Hang Lv  2 , Xiaodong Li  2 , Jie Zhang  2 , Hui Su  2 , Peiran Liu  2 , Hai Hu  2
Affiliations

Study of Guanxinning Injection on Regulatory Mechanism of Bcl-2 and Bax by Liquid Nitrogen Freezing-Mediated Femoral Head Necrosis

Xiaofeng Zhang et al. Evid Based Complement Alternat Med. 2017.

Abstract

The objective of this study was to investigate the impact of femoral head perfusion by traditional Chinese medicine Guanxinning injection promoting blood circulation for removing blood stasis on the expression of Bcl-2 and Bax induced by liquid nitrogen freezing-mediated femoral head necrosis. 90 rabbits were randomized into three groups. Normal control group was not subjected to any medication. Saline and Guanxinning group were perfused with 0.9% saline and Guanxinning injection once every three days through the hip joint, respectively. Six animals in each group were sacrificed at weeks 1, 3, 6, 9, and 12. PCR and Western blot measured the expressions of Bcl-2 and Bax in the femoral head. The bax expression in the Guanxinning group reduced at the third week significantly compared to the normal control group (P < 0.01). The Bcl-2 expression in the Guanxinning group increased substantially at the third week (P < 0.05 or P < 0.01). Prolonged treatment elevated the expression of Bcl-2 in the Guanxinning group while that of Bax reduced remarkably (P < 0.01). Moreover, the ratio of Bcl-2 to Bax increased gradually in the Guanxinning group with prolonged drug administration. Guanxinning injection can inhibit the cell apoptosis of femoral head necrosis through the treatment by femoral head perfusion.

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Figures

Figure 1
Figure 1
Expression of Bcl-2 mRNA of the femoral head in the normal control group, model group, and Guanxinning group at weeks 1, 3, 6, 9, and 12 after the treatment. (Expression of Bcl-2 mRNA in the normal control group at week 1 after the treatment is normalized to 1, and the legend shows the ratio of Bcl-2 mRNA in the normal control group to that in the other groups.)
Figure 2
Figure 2
Expression of Bax mRNA of the femoral head in the normal control group, model group, and the Guanxinning group at weeks 1, 3, 6, 9, and 12 after the treatment. (Expression of Bax mRNA in the normal control group at week 1 after the treatment is normalized to 1, and the legend shows the ratio of Bax mRNA expression in the normal control group to that in the other groups.)
Figure 3
Figure 3
The ratio of Bcl-2 to Bax of the femoral head in the normal control group, model group, and the Guanxinning group at weeks 1, 3, 6, 9, and 12 after the treatment. (The ratio of Bcl-2 to Bax in the normal control group at week 1 after the treatment is normalized to 1, and the legend shows the ratios of Bcl-2 to Bax in this group and the other groups.)
Figure 4
Figure 4
Expression of the Bcl-2 protein of the femoral head of rabbits in different groups at different time points. Lane 1 is the normal control group. Lane 2, lane 3, and lane 4 represent the model group, the control group, and the experimental group at week 1, respectively. Lane 5, lane 6, and lane 7 represent the model group, the control group, and the experimental group at week 3, respectively. Lane 8, lane 9, and lane 10 represent the model group, the control group, and the experimental group at week 6, respectively. Lane 11, lane 12, and lane 13 represent the model group, the control group, and the experimental group at week 9, respectively. Lane 14, lane 15, and lane 16 represent the model group, the control group, and the experimental group at week 12, respectively.
Figure 5
Figure 5
Expression of Bax protein of the femoral head of rabbits in different groups at various time points. Lane 1 is the normal control group. Lane 2, lane 3, and lane 4 are the model group, the control group, and the experimental group at week 1, respectively. Lane 5, lane 6, and lane 7 represent the model group, the control group, and the experimental group at week 3, respectively. Lane 8, lane 9, and lane 10 represent the model group, the control group, and the experimental group at week 6, respectively. Lane 11, lane 12, and lane 13 are the model group, the control group, and the experimental group at week 9, respectively. Lane 14, lane 15, and line 16 represent the model group, the control group, and the experimental group at week 12, respectively.
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