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. 2017 May 18;10(5):705-710.
doi: 10.18240/ijo.2017.05.07. eCollection 2017.

The expressions of metadherin and LEF-1 in mucosa-associated lymphoid tissue lymphoma of ocular adnexal

Dan Qi  1 , Hong Lin  1 , Yan Gao  1 , Jing Lin  1 , Li-Ting Hu  1 , Gui-Qiu Zhao  1
Affiliations

The expressions of metadherin and LEF-1 in mucosa-associated lymphoid tissue lymphoma of ocular adnexal

Dan Qi et al. Int J Ophthalmol. .

Abstract

Aim: To investigate the expressions of metadherin (astrocyte elevated gene-1, AEG-1) and lymphoid enhancer-binding factor-1 (LEF-1) in ocular adnexal mucosa-associated lymphoid tissue (MALT) lymphoma.

Methods: The expressions of AEG-1 and LEF-1 were detected on specimens harvested from patients suffering from MALT lymphoma and lymphadenosis of ocular adnexal in Ophthalmology Department, Affiliated Hospital of Qingdao University from 2000 to 2015 by immunohistochemical and polymerase chain reaction (PCR) analysis.

Results: AEG-1 and LEF-1 expressions in MALT lymphoma was respectively higher than that in lymphadenosis, both by immunohistochemical and PCR analysis (P<0.05). Diversity of AEG-1 and LEF-1 expressions in different Ann Arbor clinical stages showed a statistically significant result (P<0.05). A positive relevance between AEG-1 and LEF-1 was observed in MALT ocular adnexal lymphoma (r=0.435, P=0.016).

Conclusion: The over expressions of AEG-1 and LEF-1 at the level of protein and mRNA participates in the tumorigenesis of ocular adnexal MALT lymphoma. They should act as a new biological marker for pathological diagnosis in the future.

Keywords: astrocyte elevated gene-1; lymphoid enhancer-binding factor-1; ocular adnexal lymphoma.

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Figures

Figure 1
Figure 1. AEG-1 and LEF-1 mRNA expression in reactive lymphoid hyperplasia and MALT lymphoma
A: AEG-1 mRNA expression in MALT lymphoma was higher than that in reactive lymphoid hyperplasia, significantly (P<0.01); B: LEF-1 mRNA expression in MALT lymphoma was marked higher than reactive lymphoid hyperplasia (P<0.001).
Figure 2
Figure 2. HE staining of reactive lymphoid hyperplasia and MALT lymphoma
A: A compound of plasma cells, histiocytes, and small lymphocytes and a sporadic network of thin-walled capillaries were seen in reactive lymphoid hyperplasia (×400); B: The image of MALT ocular adnexal lymphoma consist of monotonous pattern of small cell lymphocytes with round or irregular nuclei and clustered chromatin, unconspicuous nucleoli and deficient cytoplasm (×400).
Figure 3
Figure 3. AEG-1 expression in reactive lymphoid hyperplasia and MALT lymphoma
A: No expression of AEG-1 in either cytoplasm or nucleus of reactive lymphoid hyperplasia (×400); B: Cytoplasm weak expression of AEG-1 in MALT, with many little brown yellow particles (×400); C: Cytoplasm strong expression of AEG-1 in MALT lymphoma (×400).
Figure 4
Figure 4. LEF-1 expression in reactive lymphoid hyperplasia and MALT lymphoma
A: No expression of LEF-1 in reactive lymphoid hyperplasia (×400); B: LEF-1 nucleus weak expression in MALT lymphoma (×400); C: LEF-1 nucleus strong expression in MALT lymphoma (×400).
See this image and copyright information in PMC

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