Soil nitrogen form and plant nitrogen uptake along a boreal forest productivity gradient

Abstract

We present results from a study of soil solution concentrations of ammonium (NH₄⁺), nitrate (NO₃^-), and amino acid N over one growing season along a local 90-m-long plant productivity gradient in a boreal forest. Three forest types are found along the gradient: an ericaceous dwarf-shrub type between 0 and 40 m, a low-herb type between 40 and 80 m, and a tall-herb type at 90 m. Soil sampling of the mor layer was performed in June, July, August and October in the three forest types. In addition, plant uptake of NH₄⁺, NO₃^- and the amino acid glycine was investigated. A mixture of the three N forms was injected into the soil; one N form at a time was labeled with ¹⁵N, and in the case of glycine also with ¹³C. In the dwarf-shrub forest, where plant productivity was low, the soil N pool was strongly dominated by amino acid N. There, plants took up more NH₄⁺ than NO₃^-. Glycine uptake did not differ significantly from either NH₄⁺ or NO₃^- uptake. Along the gradient, soil concentrations of NH₄⁺ and NO₃^- increased, as did plant productivity. In the low-herb forest NH₄⁺ comprised a major portion of the soil N pool, and plants took up more NH₄⁺ than NO₃^- or glycine. In the tall-herb forest, NO₃^- was as abundant as NH₄⁺, and together these two N forms dominated the soil N pool. Here, plants took up nearly equal amounts of NO₃^- and NH₄⁺, and this uptake exceeded that of glycine severalfold. Apart from the overall preference for NH₄⁺ that plants exhibited throughout the gradient, the results show a correlation between soil concentrations of amino acids and NO₃^- and plant preferences for these N forms.

Keywords: Amino acids; Ammonium; Glycine; Nitrate; Nitrogen.