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. 2017 May 29;15(6):152.
doi: 10.3390/md15060152.

Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical Characterization

Leto-Aikaterini Tziveleka  1 , Efstathia Ioannou  2 , Dimitris Tsiourvas  3 , Panagiotis Berillis  4 , Evangelia Foufa  5 , Vassilios Roussis  6
Affiliations

Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical Characterization

Leto-Aikaterini Tziveleka et al. Mar Drugs. .

Abstract

In search of alternative and safer sources of collagen for biomedical applications, the marine demosponges Axinella cannabina and Suberites carnosus, collected from the Aegean and the Ionian Seas, respectively, were comparatively studied for their insoluble collagen, intercellular collagen, and spongin-like collagen content. The isolated collagenous materials were morphologically, physicochemically, and biophysically characterized. Using scanning electron microscopy and transmission electron microscopy the fibrous morphology of the isolated collagens was confirmed, whereas the amino acid analysis, in conjunction with infrared spectroscopy studies, verified the characteristic for the collagen amino acid profile and its secondary structure. Furthermore, the isoelectric point and thermal behavior were determined by titration and differential scanning calorimetry, in combination with circular dichroism spectroscopic studies, respectively.

Keywords: Axinella cannabina; Suberites carnosus; marine collagen; sponges.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
SEM micrographs of insoluble collagen (InSC; A,E), intercellular collagen (ICC; B,C,F,G), and spongin-like collagen (SlC; D,H) from A. cannabina (row 1) and S. carnosus (row 2), respectively. TEM micrographs of insoluble collagen before (InSC; I,M) and after (SF-InSC; J,N) spicule removal, intercellular collagen (ICC; K,O) and spongin-like collagen (SlC; L,P) from A. cannabina (row 3) and S. carnosus (row 4), respectively.
Figure 2
Figure 2
IR spectra of insoluble collagen before (InSC; upper) and after (SF-InSC; lower) spicule removal isolated from A. cannabina (A) and S. carnosus (B).
Figure 3
Figure 3
Titration curves of insoluble collagen after spicules removal (SF-InSC) isolated from A. cannabina (A) and S. carnosus (B).
Figure 4
Figure 4
Temperature modulated DSC data of intercellular collagen (ICC) isolated from A. cannabina (A) and S. carnosus (B). The total (---), non-reversing (―) and reversing heat (-∙-) flows are presented (curves are shifted vertically for clarity).
Figure 5
Figure 5
CD spectra in the region of 190–250 nm (recorded at 20 °C) and temperature effect on the CD spectra at 221 nm of intercellular collagen (ICC) isolated from A. cannabina ((A) and (C), respectively) and S. carnosus ((B) and (D), respectively).
See this image and copyright information in PMC

References

    1. Gelse K., Pöschl E., Aigner T. Collagens-structure, function, and biosynthesis. Adv. Drug Deliv. Rev. 2003;55:1531–1546. doi: 10.1016/j.addr.2003.08.002. - DOI - PubMed
    1. Myllyharju J., Kivirikko K.I. Collagens, modifying enzymes and their mutations in humans, flies and worms. Trends Genet. 2004;20:33–43. doi: 10.1016/j.tig.2003.11.004. - DOI - PubMed
    1. Silva T.H., Moreira-Silva J., Marques A.L.P., Domingues A., Bayon Y., Reis R.L. Marine origin collagens and its potential applications. Mar. Drugs. 2014;12:5881–5901. doi: 10.3390/md12125881. - DOI - PMC - PubMed
    1. Meena C., Mengi S.A., Deshpande S.G. Biomedical and industrial applications of collagen. J. Chem. Sci. 1999;111:319–329. doi: 10.1007/BF02871912. - DOI
    1. Berillis P. Marine collagen: Extraction and applications. In: Madhukar S., editor. Research Trends in Biochemistry, Molecular Biology and Microbiology. SM Group; Dover, DE, USA: 2015. pp. 1–13.

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