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. 2017 May;54(6):1628-1637.
doi: 10.1007/s13197-017-2594-8. Epub 2017 Mar 27.

The effects of certain enzymes on the rheology of dough and the quality characteristics of bread prepared from wheat meal

Affiliations

The effects of certain enzymes on the rheology of dough and the quality characteristics of bread prepared from wheat meal

Burak Altınel et al. J Food Sci Technol. 2017 May.

Abstract

This study was carried out to evaluate the effects of amyloglucosidase, glucose oxidase, hemicellulase (mainly consist of endo-1,4-β-xylanase), cellulase, lipase, and the combination of phospholipase and hemicellulase (phospholipase + hemicellulase) on the extensographic properties of dough and the quality characteristics of bread prepared from wheat meal. The enzymes were added separately in two different amounts. The addition of glucose oxidase (at 0.0003-0.001%) caused a significant decrease in the resistance to extension, ratio of resistance to extensibility and energy values of the wheat meal dough compared with the control dough. The addition of hemicellulase (at 0.001-0.005%) and phospholipase + hemicellulase (at 0.0006-0.0009%) also improved the wheat meal dough rheology by reducing the resistance to extension and the ratio of resistance to extensibility. Glucose oxidase (at 0.0003-0.001%), hemicellulase (at 0.001-0.005%) and phospholipase + hemicellulase (at 0.0006-0.0009%) addition improved the specific volume of wheat meal bread compared with the control bread. Increasing the dosage of glucose oxidase from 0.0003 to 0.001% caused a further increase in the specific volume of wheat meal bread. The addition of hemicellulase (at 0.001-0.005%) caused a significant decrease in the baking loss and an increase in the moisture content of wheat meal bread compared with the control bread. The addition of amyloglucosidase (at 0.000875-0.001%), lipase (at 0.0002-0.001%) and cellulase (at 0.0003-0.0005%) did not considerably affected the dough rheological and the quality characteristics of wheat meal bread.

Keywords: Bakery enzymes; Extensograph; Specific volume; Wheat meal bread; Wheat meal dough.

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Figures

Fig. 1
Fig. 1
Specific volume values of breads. WMB, wheat meal bread; WFB, wheat flour bread; (C), non-enzyme added wheat meal bread (control bread); +AGL (L), 0.000875% amyloglucosidase; +AGL (H), 0.001% amyloglucosidase; +GOX (L), 0.0003% glucose oxidase; +GOX (H), 0.001% glucose oxidase; +HML (L), 0.001% hemicellulase; +HML (H), 0.005% hemicellulase; +CLL (L), 0.0003% cellulase; +CLL (H), 0.0005% cellulase; +LPS (L), 0.0002% lipase; +LPS (H), 0.001% lipase; +PHM (L), 0.0006% phospholipase + hemicellulase; +PHM (H), 0.0009% phospholipase + hemicellulase addition (w/w). Data are expressed as mean values ± standard deviations (n = 4); mean values over the columns with different superscripts are significantly different (P < 0.05); the letter “a” denotes the highest value

References

    1. AACC International Methods . Approved methods of analysis. Methods 44-15A and 46-12. St. Paul, MN, USA: The American Association of Cereal Chemists International; 2000.
    1. Bonet A, Rosell CM, Caballero PA, Gόmez M, Pérez-Munuera I, Lluch MA. Glucose oxidase effect on dough rheology and bread quality: a study from macroscopic to molecular level. Food Chem. 2006;99:408–415. doi: 10.1016/j.foodchem.2005.07.043. - DOI
    1. Bruckner PL, Habernicht D, Carlson GR, Wichman DM, Talbert LE. Comparative bread quality of white flour and whole grain flour for hard red spring and winter wheat. Crop Sci. 2001;41:1917–1920. doi: 10.2135/cropsci2001.1917. - DOI
    1. Bucsella B, Molnár D, Harasztos AH, Tömösközi S. Comparison of the rheological and end-product properties of an industrial aleurone-rich wheat flour, whole grain wheat and rye flour. J Cereal Sci. 2016;69:40–48. doi: 10.1016/j.jcs.2016.02.007. - DOI
    1. Cauvain SP, Young LS. Baking problem solved. Cambridge: Woodhead Publisher; 2001.