Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R
- PMID: 2856
- DOI: 10.1007/BF00269416
Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R
Abstract
All Bacillus subtilis R-type strains showing the phenomena of restriction and modification contain an endonuclease that inactivates in vitro the biological activity of a variety of DNAs lacking R-specific modification, such as transfecting SPPI, SPO2 and phi105 DNA, and transforming B. subtilis 168-type DNA. The corresponding DNAs carrying R-specific modification are resistant to the enzyme. The enzyme has been purified approximately 400-fold and is essentially free from contaminating double strand-directed unspecific exo- or endonuclease activity. Only Mg2+ is required as cofactor. The substrate DNAs are cleaved at specific sites. The double-stranded fragments produced from SPP1 DNA (molecular weight 2.5 x 10(7)) have an average molecular weight of about 3 x 10(5).
Similar articles
-
Restriction and modification in B. subtilis. Nucleotide sequence recognised by restriction endonuclease R. Bsu R from strain R.Mol Gen Genet. 1975 Dec 30;143(1):25-33. doi: 10.1007/BF00269417. Mol Gen Genet. 1975. PMID: 814403
-
The restriction endonucleases in Bacillus amyloliquefaciens N strain. Substrate specificities.Biochim Biophys Acta. 1976 Aug 18;442(2):184-96. doi: 10.1016/0005-2787(76)90489-5. Biochim Biophys Acta. 1976. PMID: 182257
-
Purification and properties of a new restriction endonuclease from Haemophilus influenzae Rf.Eur J Biochem. 1978 Dec;92(2):417-26. doi: 10.1111/j.1432-1033.1978.tb12762.x. Eur J Biochem. 1978. PMID: 33045
-
["Host-controlled modification and restriction" and the relating enzymes in Bacillus subtilis and other Bacillus strains (author's transl)].Tanpakushitsu Kakusan Koso. 1977 Jul 1;22(8):1012-9. Tanpakushitsu Kakusan Koso. 1977. PMID: 412233 Review. Japanese. No abstract available.
-
Structures and mechanisms of DNA restriction and modification enzymes.Q Rev Biophys. 1979 Aug;12(3):315-69. doi: 10.1017/s0033583500005461. Q Rev Biophys. 1979. PMID: 232555 Review. No abstract available.
Cited by
-
Restriction and modification enzymes and their recognition sequences.Nucleic Acids Res. 1981 Jan 10;9(1):r75-96. doi: 10.1093/nar/9.1.213-c. Nucleic Acids Res. 1981. PMID: 6259615 Free PMC article. Review. No abstract available.
-
Restriction and modification in B. subtilis. Nucleotide sequence recognised by restriction endonuclease R. Bsu R from strain R.Mol Gen Genet. 1975 Dec 30;143(1):25-33. doi: 10.1007/BF00269417. Mol Gen Genet. 1975. PMID: 814403
-
The genome of Bacillus subtilis phage SPP1: the arrangement of restriction endonuclease generated fragments.Mol Gen Genet. 1979 Jan 10;168(2):165-72. doi: 10.1007/BF00431442. Mol Gen Genet. 1979. PMID: 109739
-
Introduction of host-controlled modification and restriction systems of Bacillus subtilis IAM1247 into Bacillus subtilis 168.J Bacteriol. 1979 Jul;139(1):308-10. doi: 10.1128/jb.139.1.308-310.1979. J Bacteriol. 1979. PMID: 110786 Free PMC article.
-
Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.Nucleic Acids Res. 1992 May 11;20 Suppl(Suppl):2145-57. doi: 10.1093/nar/20.suppl.2145. Nucleic Acids Res. 1992. PMID: 1317957 Free PMC article. No abstract available.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous
