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. 1975 Dec 30;143(1):13-23.
doi: 10.1007/BF00269416.

Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R

S Bron, K Murray, T A Trautner

Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R

S Bron et al. Mol Gen Genet. .

Abstract

All Bacillus subtilis R-type strains showing the phenomena of restriction and modification contain an endonuclease that inactivates in vitro the biological activity of a variety of DNAs lacking R-specific modification, such as transfecting SPPI, SPO2 and phi105 DNA, and transforming B. subtilis 168-type DNA. The corresponding DNAs carrying R-specific modification are resistant to the enzyme. The enzyme has been purified approximately 400-fold and is essentially free from contaminating double strand-directed unspecific exo- or endonuclease activity. Only Mg2+ is required as cofactor. The substrate DNAs are cleaved at specific sites. The double-stranded fragments produced from SPP1 DNA (molecular weight 2.5 x 10(7)) have an average molecular weight of about 3 x 10(5).

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