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. 1988 Jan;3(1):24-9.
doi: 10.3904/kjim.1988.3.1.24.

Hepatitis B virus DNA detection by in situ hybridization in human hepatocellular carcinoma

D H Lee, J D Lee

Hepatitis B virus DNA detection by in situ hybridization in human hepatocellular carcinoma

D H Lee et al. Korean J Intern Med. 1988 Jan.

Abstract

The distribution of hepatitis B virus (HBV) DNA in tumor tissue sections from six Korean patients with HBsAg positive hepatocellular carcinoma (HCC) was examined by in situ hybridization using a biotin-labeled recombinant, cloned HBV DNA probe. All patients tested were positive for both HBeAg and anti-HBc in their sera. HBV DNA was distributed abundantly in the cytoplasm and rarely in the nuclei of tumor cells. The validity of the in situ hybridization assay was confirmed by the dot blotting technique using a 32P-labeled HBV DNA probe obtained by nick translation. In conclusion, it is speculated that integration of HBV DNA into host DNA as well as persistant amplified replication of the HBV DNA within the hepatocytes is linked etiologically to the development of human hepatocellular carcinoma.

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Figures

Fig. 1.
Fig. 1.
In situ hybridization of HBV DNA in hepatocellular carcinoma. The cytoplasm of HCC show abundant HBV DNA granules. The nuclei rarely reveal DNA grains. (Methyl green counterstain, × 400)
Fig. 2.
Fig. 2.
In situ hybridization of HBV DNA in the cirrhotic areas around HCC. The cytoplasm of the cirrhotic nodule shows plentiful HBV DNA grains. (×200)
Fig. 3.
Fig. 3.
High power details of figure 2. The nuclei reveal no discernible grains of HBV DNA. (Methyl green counter stain, × 400)
Fig. 4.
Fig. 4.
Autoradiography of the dot blot hybridization of sera of patients with HCC utilizing a32P-labeled HBV DNA probe (specific activity, 106 cpm/μg DNA). To determine the concentration, the indicated amounts of unlabeled recombinant lambda-HBV DNA with serial dilutions were directly spotted on the nitrocellulose paper in 50μl volumes, followed by hybridization with labeled DNA, and then autoradiographed for signal detection.
See this image and copyright information in PMC

References

    1. Shafritz DA, Kew MC. Identification of integrated hepatitis B virus DNA sequences in human hepatocellular carcinoma. Hepatology. 1981;1:1. - PubMed
    1. Lieberman HM, LaBrecque DR, Kew MC, Hadziyannis SJ, Shafritz DA. Detection of hepatitis B virus DNA directly in human serum by a simplified molecular hybridization test: Comparison to HBeAg/anti-HBe status in HBsAg carriers. Hepatology. 1983;3:285. - PubMed
    1. Brechot C, Hadchovel M, Scotto J. Detection of hepatitis B virus DNA in liver and serum: A direct appraisal of the chronic carrier state. Lancet. 1981;2:765. - PubMed
    1. Sninsky JJ, Cohen SN. Specialized cloning vectors for hepatitis B virus gene expression in Escherichia coli. Hepatology. 1982;2:72S.
    1. Blum HE, Stowring L, Figus A, Montgomery CK, Haase AT, Vyas GN. Detection of hepatitis B virus DNA in hepatocytes, bile duct epithelium, and vascular elements by in situ hybridization. Proc Natl Acad Sci USA. 1983;80:6685. - PMC - PubMed