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. 2017 May;13(5):1789-1792.
doi: 10.3892/etm.2017.4160. Epub 2017 Feb 23.

Raman spectrum: A potential biomarker for embryo assessment during in vitro fertilization

Jiayi Ding  1 , Tian Xu  2 , Xiaofang Tan  1 , Hua Jin  1 , Jun Shao  1 , Haibo Li  1
Affiliations

Raman spectrum: A potential biomarker for embryo assessment during in vitro fertilization

Jiayi Ding et al. Exp Ther Med. 2017 May.

Abstract

The aim of the study was to investigate whether Raman spectrum is consistent with the morphological scoring of the embryo of day 3 during in vitro fertilization (IVF). The spent culture media of embryo of day 3 from 10 patients were collected and analyzed. The samples were analyzed using Raman spectroscopy and graded according to the standard embryo scoring system simultaneously. Data showed that the Raman spectra obtained from the droplet of media were useful, as they can act as the characteristic signature for protein and amino acids. The Raman biospectroscopy-based metabonomics profiling of spent media was consistent with the result of conventional morphological evaluation. In conclusion, this technology offers great potential for the development of tools allowing rapid non-invasive assessment of the quality of embryo of day 3 during IVF.

Keywords: Raman spectrum; biomarker; embryo; in vitro fertilization.

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Figures

Figure 1.
Figure 1.
Typical Raman spectrum of spent embryo media. Green and black line: Raman spectrum of spent media of high quality embryo. Red and blue line: Raman spectrum of spent media of poor quality embryo. Statistical analyses were not applied due to the limited number of patients.
Figure 2.
Figure 2.
The image of embryos of day 3 in different grade by morphological scoring. The high quality with amplification is representative for the morphopathology in cells.
See this image and copyright information in PMC

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