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. 2017 Jan 13;14(2):206-216.
doi: 10.21010/ajtcam.v14i2.22. eCollection 2017.

PROTECTIVE EFFECT OF MORINGA PEREGRINA LEAVES EXTRACT ON ACETAMINOPHEN -INDUCED LIVER TOXICITY IN ALBINO RATS

Samy Abdelfatah Abdel Azim  1 , Mohamed Taha Abdelrahem  1 , Mostafa Mohamed Said  1 , Alshaimaa Khattab  1
Affiliations

PROTECTIVE EFFECT OF MORINGA PEREGRINA LEAVES EXTRACT ON ACETAMINOPHEN -INDUCED LIVER TOXICITY IN ALBINO RATS

Samy Abdelfatah Abdel Azim et al. Afr J Tradit Complement Altern Med. .

Abstract

Background: Acetaminophen is a common antipyretic drug but at overdose can cause severe hepatotoxicity that may further develop into liver failure and hepatic centrilobular necrosis in experimental animals and humans. This study was undertaken to assess the ameliorative role of Moringa peregrina leaves extract against acetaminophen toxicity in rats.

Materials and methods: Induction of hepatotoxicity was done by chronic oral administration of acetaminophen (750 mg/kg bwt) for 4 weeks. To study the possible hepatoprotective effect, Moringa peregrina leaves extract (200 mg/kg bwt) or Silymarin (50 mg/kg bwt) was administered orally, for 4 weeks, along with acetaminophen.

Results: acetaminophen significantly increased serum liver enzymes and caused oxidative stress, evidenced by significantly increased tissue malondialdehyde, glutathione peroxidase, hepatic DNA fragmentation, and significant decrease of glutathione and antioxidant enzymes in liver, blood and brain. On the other hand, administration of Moringa peregrina leaves extract reversed acetaminophen-related toxic effects through: powerful malondialdehyde suppression, glutathione peroxidase normalization and stimulation of the cellular antioxidants synthesis represented by significant increase of glutathione, catalase and superoxide dismutase in liver, blood and brain, besides, DNA fragmentation was significantly decreased in the liver tissue.

Conclusion: acetaminophen induced oxidative damage can be improved by Moringa peregrina leaves extract-treatment, due to its antioxidant potential.

Keywords: Antioxidant; DNA fragmentation; Moringaceae; NAPQI; Oxidative stress.

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Figures

Figure 1
Figure 1
High-performance liquid chromatography (HPLC) chromatograms of MPL, at 280 nm (A) and 330 nm (B).
Figure 2
Figure 2
Percentage of DPPH inhibition against different concentrations of MPL extract and Ascorbic acid. Values are mean ± standard error of triplicate determinations.
Figure 3
Figure 3
Digital photograph of DNA electrophoresis of liver tissues shows the protective effects of different treatments against APAP toxicity, where, L1: negative control (N); L2: APAP; L3: APAP+MPL; L4: APAP+SIL; L5: APAP+SIL+MPL and M: DNA marker.
Figure 4
Figure 4
The analytical peaks for DNA lanes which resemble the same lanes in Fig. 2 using Biogene software.
See this image and copyright information in PMC

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