Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Jan 13;14(2):227-233.
doi: 10.21010/ajtcam.v14i2.24. eCollection 2017.

THERAPEUTIC POTENTIALS OF ETHANOLIC EXTRACT OF LEAVES OF HOLARRHENA FLORIBUNDA (G. DON) DUR. AND SCHINZ (APOCYNACEAE)

Yao Patrick Hoekou  1 , Tchadjobo Tchacondo  1 , Simplice Damintoti Karou  1 , Rakiswende Serge Yerbanga  2 , Elom Achoribo  3 , Ollo Da  2 , Wouyo Atakpama  4 , Komlan Batawila  4
Affiliations

THERAPEUTIC POTENTIALS OF ETHANOLIC EXTRACT OF LEAVES OF HOLARRHENA FLORIBUNDA (G. DON) DUR. AND SCHINZ (APOCYNACEAE)

Yao Patrick Hoekou et al. Afr J Tradit Complement Altern Med. .

Abstract

Background: Holarrhena floribunda is a plant of wide usage in the Togolese folk medicine. A previous ethnobotanical survey on the latex plants of the Maritime region of the country revealed that this plant was included in several recipes curing malaria and microbial infections. Therefore, this study aimed to seek for the effectiveness of the ethanolic extract of the plant in the treatment of these diseases.

Methods: The antimicrobial test was performed using the agar well-diffusion and the NCCLS broth microdilution methods, while the in vivo antimalarial activity was evaluated following the four-day suppressive test of Peters. The acute toxic effects of the extract were monitored after a single oral dose (5,000 mg/kg body weight) administration in NMRI mice.

Results: The results indicated that the ethanolic extract of leaves of H. floribunda was active on Staphylococcus aureus ATCC 29213 and clinical strains of Staphylococcus aureus, Salmonella typhi and Klebsiella pneumoniae with MICs ranging from 0.62 to 1.25 mg/mL. The extract also showed significant parasitaemia suppression in a dose-dependent manner. In the acute toxicity assay, the oral administration of the extract to the mice did not affect the relative weight of vital organs, and there were no signs of toxicity or death during the study period. The LD50 of the tested extract was found to be greater than 5,000 mg/kg, indicating its safety.

Conclusion: This study demonstrates the antibacterial and antimalarial activities of leaves of H. floribunda and then, supports its medicinal use in the treatment of microbial infections.

Keywords: Holarrhena floribunda; antibacterial; antimalarial; ethanolic extract; toxicity.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Abdulelah HAA, Zainal-Abidin BAH. In vivo antimalarial tests of Nigella sativa (Black Seed) different extracts. Am. J. Pharm. & Toxicol. 2007;2:46–50.
    1. Arbonnier M. Arbres, arbustes et lianes des zones sèches d’Afrique de l’Ouest. 2emeéd. CIRAD-MNHN. 2000:541.
    1. Badmus JA, Odunola OA, Yekeen TA, Gbadegesin AM, Fatoki JO, Godo MO, Oyebanjo KS, Hiss DC. Evaluation of antioxidant, antimutagenic, and lipid peroxidation inhibitory activities of selected fractions of Holarrhenafloribunda (G. Don) leaves. Acta Biochim. Pol. 2013;60(3):435–442. - PubMed
    1. Bayala B, Tamboura HH, Pellicer MTR, Zongon D, Traoré A, Ouédraogo L, Malpaux B, Sawadogo L. Effets oestrogéniques du macéréaqueux des feuilles de Holarrhena floribunda (G. Don). Dur & Schinz chez la rate ovariectomisée. Biotechn. Agr. Soc. Env. 2006;10:173–180.
    1. Berhaut J. Dicotylédones. Volume 1. Acanthacées àAvicenniacées. Gouvernement du Sénégal, Ministère du Développement Rural et de l’Hydraulique, Direction des Eaux et Forets. Dakar, Sénégal: 1971. Flore illustrée du Sénégal; p. 626.

MeSH terms

LinkOut - more resources