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. 2017 Jan 13;14(2):311-318.
doi: 10.21010/ajtcam.v14i2.32. eCollection 2017.

IN VITRO STUDY OF CONCENTRATION-EFFECT AND TIME-COURSE PATTERN OF WHITE ALUM ON ESCHERICHIA COLI O157:H7 GROWTH

Reza Shahriari  1 , Saeed Salari  1 , Saeed Shahriari  1
Affiliations

IN VITRO STUDY OF CONCENTRATION-EFFECT AND TIME-COURSE PATTERN OF WHITE ALUM ON ESCHERICHIA COLI O157:H7 GROWTH

Reza Shahriari et al. Afr J Tradit Complement Altern Med. .

Abstract

Background: Nowadays, the demand for antibacterial fabrics has increased. White alum is used for oral aphthous ulcers treatment in traditional medicine of Sistan city, Sistan and Baluchistan Province, Iran, and also as a flocculent for water purification. This study is aimed to evaluate the effect of concentration and time on antibacterial activity of white alum on Escherichia coli O157:H7.

Materials and methods: 0.25, 0.5, 1 and 2% concentrations of white alum were added to 108 CFU of Escherichia coli O157:H7. Optical Density was recorded for 4 hours. Data obtained were analyzed using Repeated Measure and One-way ANOVA by SPSS.

Results: Results revealed the effectiveness of white alum in the growth of the tested bacterium. The white alum was found to be potent against Escherichia coli O157:H7 at a concentration above 1% (p<0.05). Also, its effect is dose and time dependent, as well as other disinfectants.

Conclusion: A wide variety of natural products has been under scrutiny for their clinical potential, both in terms of prevention and treatment. Strong antibacterial activity of white alum compared with control was shown against tested bacterium. In conclusion, white alum can be used as an inhibitor of bacterial growth, especially for Escherichia coli O157:H7.

Keywords: Antibacterial activity; Escherichia coli O157:H7; Sistan; Steoptria Alon aloman alen; White alum.

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Figures

Figure 1
Figure 1
Growth of E. coli O157:H7 mixed with different concentrations of white alum during 4 hours. Error Bars display Confidence Interval for Mean. Asterisks indicate the signficance difference, compared with BAC+PW (p<0.05).
Figure 2
Figure 2
Growth of E. coli O157:H7 mixed with different concentrations of white alum after 0.5, 1, 1.5, 52, 2.5, 3, 3.5 and 4 hour. BAC+PW: tested bacterium incubated with pepton water broth; BAC+Z0.25: tested bacterium incubated with %0.25 white alum; BAC+Z0.5: tested bacterium incubated with %0.5 white alum, BAC+Z01: tested bacterium incubated with %1 white alum, BAC+Z2: tested bacterium incubated with %2 white alum.
Figure 3
Figure 3
Microtitre plate at the outset of investigation loading with E. coli O157:H7 mixed with white alum. A, C and E rows represent the repetition; Column 5, 10 and 12 were remained empty to increase accuracy.
Figure 4
Figure 4
pectrophotometry of mixture of the tested bacterium and white alum in beginning of experiment.
Figure 5
Figure 5
Microtitre plate after 4 hours.
Figure 6
Figure 6
Spectrophotometry after 4 hours.
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References

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