doi: 10.1007/978-1-4939-7116-9_6.
Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems
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- PMID: 28573612
- DOI: 10.1007/978-1-4939-7116-9_6
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Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems
Methods Mol Biol.
2017.
Abstract
In this chapter, we describe the minigenome system for Ebola virus (EBOV), which reconstitutes EBOV polymerase activity in cells and can be used to model viral genome replication and transcription. This protocol comprises all steps including cell culture, plasmid preparation, transfection, and luciferase reporter assay readout.
Keywords: Ebola virus; Filoviruses; Firefly luciferase assay; Minigenome; Replication; Replication promoter; Reporter gene; Transcription; Zaire ebolavirus; β-galactosidase enzyme assay.
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