In vitro and in vivo evaluation of cubosomal in situ nasal gel containing resveratrol for brain targeting

Abstract

The aim of this study was to enhance the delivery of resveratrol to the brain through the transnasal route by cubosomes. Cubosomes were prepared using glycerol monooleate and Lutrol F127 by probe sonication method. A 3² full factorial design was used for optimization of cubosomes and batch containing 4% w/v glycerol monooleate and 1.5% w/v of Lutrol F 127 was optimized. The selected cubosomal batch was cubical in shape, having mean particle size 161.5 ± 0.12 nm. Entrapment efficiency was found to be 83.08% with zeta potential of -20.9 mV. In vitro release of cubosomal batch showed controlled release of drug profile (67%) up to 24 h. The optimized cubosomal dispersion was dispersed into gelling polymer (poloxamer 407) to form in situ gel for nasal use. The optimal cubosomal gel (containing 12% w/v poloxamer 407) had been subjected to ex-vivo permeation and in vivo biodistribution studies. It showed significantly higher transnasal permeation and better distribution to brain, when compared to the drug solution (i.n.) and drug solution (oral). Finally the cubosomal gel could be considered as a promising carrier for brain targeting of Resveratrol (Res) through transnasal route.

Keywords: In vivo biodistribution; Resveratrol; brain targeting; cubosomes; transnasal.