Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Jun 4:23:2721-2731.
doi: 10.12659/msm.905064.

Genome-Wide Profiling of miRNA and mRNA Expression in Alzheimer's Disease

Wan-Sheng Chang  1 , Yong-Hong Wang  1 , Xiao-Tun Zhu  1 , Chuan-Jie Wu  2
Affiliations

Genome-Wide Profiling of miRNA and mRNA Expression in Alzheimer's Disease

Wan-Sheng Chang et al. Med Sci Monit. .

Abstract

BACKGROUND Our study aimed to identify key differentially expressed genes (DEGs) and miRNAs (DEmiRNAs) which can serve as potential biomarkers for diagnosis and therapy of Alzheimer's disease (AD). MATERIAL AND METHODS We performed miRNA and mRNA integrated analysis (MMIA) to identify DEGs and DEmiRNAs of AD. The AD-specific DEmiRNAs-targets interaction network was contrasted. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were performed. Q-RT-PCR was used to verify the expression of selected DEGs and DEmiRNAs. RESULTS We conducted MMIA of AD based on 1 miRNA dataset and 3 mRNA datasets derived from the Gene Expression Omnibus (GEO) database; 1759 DEGs and 12 DEmiRNAs were obtained. DEGs of AD were significantly enriched in Huntington's disease and AD. LRP1, CDK5R1, PLCb2, NDUFA4, and DLG4 were 5 DEGs regulated by 4 DEmiRNAs, including miR-26b-5p, miR-26a-5p, miR-107, and miR-103a-3p. These 4 miRNAs were the top 4 miRNAs covering most DEGs. According to the qRT-PCR results, the expression of PLCβ2, NDUFA4, DLG4, miR-107, and miR-103a-3p was consistent with our integrated analysis. CONCLUSIONS We concluded that LRP1, CDK5R1, PLCβ2, NDUFA4, and DLG4 may play a role in AD regulated by miR-26b-5p, miR-26a-5p, miR-107, and miR-103a-3p. Our findings will contribute to identification of biomarkers and new strategies for drug design for AD treatment.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest

The authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
Heat-map image displaying the top 100 genes that were significantly up-regulated or down-regulated (P-value <0.05) in AD compared to normal controls.
Figure 2
Figure 2
AD-specific DEmiRNA-mRNA interaction network. The green rectangle represents down-regulation of miRNAs, and the red ellipse represents the up-regulation of target genes. The arrow lines indicate miRNAs-targets pairs with negative correlations.
Figure 3
Figure 3
Pathway of Huntington’s disease enriched in target DEGs of DEmiRNAs of AD. The red rectangles represent the elements regulated by the target DEGs of DEmiRNAs that are enriched in Huntington’s disease.
Figure 4
Figure 4
Pathway of AD enriched in target DEGs of DEmiRNAs of AD. The red rectangles represent the elements regulated by target DEGs of DEmiRNAs enriched in AD.
Figure 5
Figure 5
QRT-PCR results of DEmiRNAs and target DEGs in AD.
See this image and copyright information in PMC

References

    1. Brookmeyer R, Johnson E, Ziegler-Graham K, Arrighi HM. Forecasting the global burden of Alzheimer’s disease. Alzheimers Dement. 2007;3:186–91. - PubMed
    1. Frisoni GB, Pievani M, Testa C, et al. The topography of grey matter involvement in early and late onset Alzheimer’s disease. Brain. 2007;130:720–30. - PubMed
    1. Olsen I, Singhrao SK. Can oral infection be a risk factor for Alzheimer’s disease? J Oral Microbiol. 2015;7:29143. - PMC - PubMed
    1. Satoh J, Kino Y, Niida S. MicroRNA-Seq data analysis pipeline to identify blood biomarkers for Alzheimer’s disease from public data. Biomark Insights. 2015;10:H1–H6. - PMC - PubMed
    1. Maloney B, Lahiri DK. The Alzheimer’s amyloid β-peptide (Aβ) binds a specific DNA Aβ-interacting domain (AβID) in the APP, BACE1, and APOE promoters in a sequence-specific manner: Characterizing a new regulatory motif. Gene. 2011;488:1–12. - PMC - PubMed