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. 2017 Jun 2;17(1):144.
doi: 10.1186/s12872-017-0570-x.

Macrophage migration inhibitory factor promoter polymorphisms (-794 CATT5-8): Relationship with soluble MIF levels in coronary atherosclerotic disease subjects

Lu Qian  1 , Xiao-Yan Wang  2 , Saroj Thapa  1 , Lu-Yuan Tao  1 , Shao-Ze Wu  1 , Gao-Jiang Luo  2 , Lu-Ping Wang  1 , Jiao-Ni Wang  1 , Jie Wang  1 , Ji Li  1 , Ji-Fei Tang  3 , Kang-Ting Ji  4
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Macrophage migration inhibitory factor promoter polymorphisms (-794 CATT5-8): Relationship with soluble MIF levels in coronary atherosclerotic disease subjects

Lu Qian et al. BMC Cardiovasc Disord. .

Abstract

Background: We analyzed the relationship of -794 CATT5-8 MIF polymorphisms with soluble MIF in Coronary Atherosclerotic Disease (CAD) patients.

Methods: A total of 256 patients selected, on which 186 normal-coronary and 70 Coronary artery disease subjects, were recruited in the study (Retrospectively registered). Genotyping of -794 CATT5-8 polymorphisms were performed by PCR and DNA sequencing. Serum MIF levels were measured using an ELISA kit. Patients were classified by coronary angiogram, and CAD based on Gensini's integral degree (angiographic scoring system).

Results: The allele frequency and genotype frequency of -794 CATT5-8 did not show any differences in normal-coronary subjects and CAD subjects. In CAD patients, serum MIF levels was lower in CATT (5) subjects than in CATT (7) subjects, while the genotype of -794 CATT5-8 did not show differences in serum MIF levels. In addition, we found a decrease in serum MIF levels in carriers of the (5/5) genotypes the -794 CATT5-8 MIF polymorphisms, although it was not significant. There was no relationship of CAD class and the allele frequency of -794 CATT5-8.

Conclusions: This study found no association between CAD class and -794 CATT5-8 MIF polymorphisms with soluble MIF levels in CAD Subjects.

Trial registration: NCT01750502 (November 2012, Retrospectively registered).

Keywords: Coronary Atherosclerotic Disease; Gene polymorphisms; Gensini’s degree integral; Macrophage Migration Inhibitory Factor.

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Figures

Fig. 1
Fig. 1
the plasma MIF concentration of carriers of MIF −794CATT5–8 alleles and genotypes in CAD patients. a The plasma MIF concentration of the carriers of CATT(5) allele was significantly lower than that of the CATT(7) allele carriers (P < 0.05). b The plasma MIF concentration was lower in the CATT(5/5) group than CATT(6/6) and CATT(7/7) groups but the difference was not statically significant (P > 0.05)
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