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. 2017 Spring;19(Suppl 1):37-43.
doi: 10.22074/cellj.2017.4705. Epub 2017 May 17.

Enterolactone Reduces Telomerase Activity and The Level of Its Catalytic Subunit in Breast Cancer Cells

Davod Ilbeigi  1 , Mitra Nourbakhsh  2   3 , Shahnaz Khaghani  1 , Nahid Einollahi  4 , Nejat Kheiripour  1 , Zafar Gholinejad  1 , Mohammad Alaee  1 , Mostafa Saberian  4
Affiliations

Enterolactone Reduces Telomerase Activity and The Level of Its Catalytic Subunit in Breast Cancer Cells

Davod Ilbeigi et al. Cell J. 2017 Spring.

Abstract

Objective: There is a positive correlation between higher serum phytoestrogen concentrations and lower risk of breast cancer. The activation of telomerase is crucial for the growth of cancer cells; therefore, the aim of this study was to examine the effects of enterolactone (ENL) and enterodiol (END) on this enzyme.

Materials and methods: In this experimental study, we performed the viability assay to determine the effects of different concentrations of ENL and END on cell viability, and the effective concentrations of these two compounds on cell growth. We used western blot analysis to evaluate human telomerase reverse transcriptase catalytic subunit (hTERT) expression and polymerase chain reaction (PCR)-ELISA based on the telomeric repeat amplification protocol (TRAP) assay for telomerase activity.

Results: Both ENL and END, at 100 μM concentrations, significantly (P<0.05) reduced cell viability. However, only the 100 μM concentration of ENL significantly (P<0.05) decreased hTERT protein levels and telomerase activity. Lower concentrations of ENL did not have any significant effects on telomerase activity and hTERT protein levels.

Conclusion: High concentration of ENL decreased the viability of MCF-7 breast cancer cells and inhibited the expression and activity of telomerase in these cells. Although END could reduce breast cancer cell viability, it did not have any effect on telomerase expression and activity.

Keywords: Breast Cancer; Enterodiol; Enterolactone; Lignan; Telomerase.

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Figures

Fig.1
Fig.1
The effects of different concentrations of A. Enterolactone (ENL) and B. Enterodiol (END) on MCF-7 cell viability, determined by the MTT assay. Data are shown as percentage of control cells compared by one-way analysis of variance (ANOVA) and are the means of at least three separate experiments. *; Significant decrease compared to control P<0.05.
Fig.2
Fig.2
Enterolactone (ENL), but not enterodiol (END), decreased human telomerase reverse transcriptase catalytic subunit (hTERT) expression. Western blot analysis of hTERT protein levels in response to different concentrations of A. ENL and17β- estradiol (E2), and B. END and E2 for 48 hours. The values are the means of results of at least three separate experiments compared by one-way analysis of variance (ANOVA), and C. A representative western blotting of hTERT protein levels in MCF-7 cells after treatment with ENL, END, and E2. *; P<0.05.
Fig.3
Fig.3
The effects of different concentrations of A. Enterolactone (ENL) and B. Enterodiol (END) on telomerase enzymatic activity in MCF-7 cells. Only the 100 µM concentration of ENL significantly reduced telomerase activity. Each value is the mean of three separate experiments performed in duplicates and compared by one-way analysis of variance (ANOVA). *; P<0.05.
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