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. 2017 Jul;14(7):687-690.
doi: 10.1038/nmeth.4324. Epub 2017 Jun 5.

Differential analysis of RNA-seq incorporating quantification uncertainty

Harold Pimentel  1 , Nicolas L Bray  2 , Suzette Puente  3 , Páll Melsted  4 , Lior Pachter  5
Affiliations
Free article

Differential analysis of RNA-seq incorporating quantification uncertainty

Harold Pimentel et al. Nat Methods. 2017 Jul.
Free article

Abstract

We describe sleuth (http://pachterlab.github.io/sleuth), a method for the differential analysis of gene expression data that utilizes bootstrapping in conjunction with response error linear modeling to decouple biological variance from inferential variance. sleuth is implemented in an interactive shiny app that utilizes kallisto quantifications and bootstraps for fast and accurate analysis of data from RNA-seq experiments.

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