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. 2017 May 29;84(1):e1-e6.
doi: 10.4102/ojvr.v84i1.1411.

Detection and prevalence of antimicrobial resistance genes in Campylobacter spp. isolated from chickens and humans

Samantha Reddy, Oliver T Zishiri  1
Affiliations

Detection and prevalence of antimicrobial resistance genes in Campylobacter spp. isolated from chickens and humans

Samantha Reddy et al. Onderstepoort J Vet Res. .

Abstract

Campylobacter spp. are common pathogenic bacteria in both veterinary and human medicine. Infections caused by Campylobacter spp. are usually treated using antibiotics. However, the injudicious use of antibiotics has been proven to spearhead the emergence of antibiotic resistance. The purpose of this study was to detect the prevalence of antibiotic resistance genes in Campylobacter spp. isolated from chickens and human clinical cases in South Africa. One hundred and sixty one isolates of Campylobacter jejuni and Campylobacter coli were collected from chickens and human clinical cases and then screened for the presence of antimicrobial resistance genes. We observed a wide distribution of the tetO gene, which confers resistance to tetracycline. The gyrA genes that are responsible quinolone resistance were also detected. Finally, our study also detected the presence of the blaOXA-61, which is associated with ampicillin resistance. There was a higher (p < 0.05) prevalence of the studied antimicrobial resistance genes in chicken faeces compared with human clinical isolates. The tetO gene was the most prevalent gene detected, which was isolated at 64% and 68% from human and chicken isolates, respectively. The presence of gyrA genes was significantly (p < 0.05) associated with quinolone resistance. In conclusion, this study demonstrated the presence of gyrA (235 bp), gyrA (270 bp), blaOXA-61 and tetO antimicrobial resistance genes in C. jejuni and C. coli isolated from chickens and human clinical cases. This indicates that Campylobacter spp. have the potential of resistance to a number of antibiotic classes.

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Conflict of interest statement

The authors declare that they have no financial or personal relationships that may have inappropriately influenced them in writing this article.

Figures

FIGURE 1
FIGURE 1
Representative gel of antibiotic resistance genes investigated from Campylobacter spp.
FIGURE 2
FIGURE 2
Percentage of Campylobacter jejuni and Campylobacter coli, from human clinical isolates (n = 83) and chicken faeces (n = 78) that resulted positive to each of the four antibiotic resistance genes under analysis.
See this image and copyright information in PMC

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