Uptake, transport and distribution of molybdenum in two oilseed rape (Brassica napus L.) cultivars under different nitrate/ammonium ratios

Abstract

Objectives: To investigate the effects of different nitrate sources on the uptake, transport, and distribution of molybdenum (Mo) between two oilseed rape (Brassica napus L.) cultivars, L0917 and ZS11.

Methods: A hydroponic culture experiment was conducted with four nitrate/ammonium (NO₃^-:NH₄⁺) ratios (14:1, 9:6, 7.5:7.5, and 1:14) at a constant nitrogen concentration of 15 mmol/L. We examined Mo concentrations in roots, shoots, xylem and phloem sap, and subcellular fractions of leaves to contrast Mo uptake, transport, and subcellular distribution between ZS11 and L0917.

Results: Both the cultivars showed maximum biomass and Mo accumulation at the 7.5:7.5 ratio of NO₃^-:NH₄⁺ while those were decreased by the 14:1 and 1:14 treatments. However, the percentages of root Mo (14.8% and 15.0% for L0917 and ZS11, respectively) were low under the 7.5:7.5 treatment, suggesting that the equal NO₃^-:NH₄⁺ ratio promoted Mo transportation from root to shoot. The xylem sap Mo concentration and phloem sap Mo accumulation of L0917 were lower than those of ZS11 under the 1:14 treatment, which suggests that higher NO₃^-:NH₄⁺ ratio was more beneficial for L0917. On the contrary, a lower NO₃^-:NH₄⁺ ratio was more beneficial for ZS11 to transport and remobilize Mo. Furthermore, the Mo concentrations of both the cultivars' leaf organelles were increased but the Mo accumulations of the cell wall and soluble fraction were reduced significantly under the 14:1 treatment, meaning that more Mo was accumulated in organelles under the highest NO₃^-:NH₄⁺ ratio.

Conclusions: This investigation demonstrated that the capacities of Mo absorption, transportation and subcellular distribution play an important role in genotype-dependent differences in Mo accumulation under low or high NO₃^-:NH₄⁺ ratio conditions.

Keywords: Brassica napus L.; Nitrogen source; Transport; Subcellular distribution; Xylem; Phloem.

Fig. 1

Fresh weights of roots (a) and shoots (b) at different NO₃ ⁻:NH₄ ⁺ ratios in hydroponic culture Data were presented as mean±SE (n=4). Different letters above the bar mean significant differences at P<0.05 by LSD-test

Fig. 2

Total plant Mo accumulations (a) and tissue Mo accumulation percentages (b) at different NO₃ ⁻:NH₄ ⁺ ratios in hydroponic culture Data were presented as mean±SE (n=4). Different letters above the bar mean significant differences at P<0.05 by LSD-test

Fig. 3

Mo concentrations in roots (a) and shoots (b) of oilseed rape grown at different NO₃ ⁻:NH₄ ⁺ ratios in hydroponic culture Data were presented as mean±SE (n=4). Different letters above the bar mean significant differences at P<0.05 by LSD-test

Fig. 4

Mo translocation factors (TFs) for shoot/root of L0917 (a) and ZS11 (b) at different NO₃ ⁻:NH₄ ⁺ ratios in hydroponic culture Data were presented as mean±SE (n=4). Different letters above the bar mean significant differences at P<0.05 by LSD-test

Fig. 5

Mo concentration in xylem sap (a) and Mo accumulation in phloem sap (b) of oilseed rape grown at different NO₃ ⁻:NH₄ ⁺ ratios in hydroponic culture Data were presented as mean±SE (n=4). Different letters above the bar mean significant differences at P<0.05 by LSD-test

Fig. 6

Mo concentrations in cell wall (a), organelle (b), soluble fractions (c) of oilseed rape grown at different NO₃ ⁻:NH₄ ⁺ ratios in hydroponic culture Data were presented as mean±SE (n=4). Different letters above the bar mean significant differences at P<0.05 by LSD-test

Fig. 7

Mo percentages in subcellular fractions for L0917 (a) and ZS11 (b) at different NO₃ ⁻:NH₄ ⁺ ratios in hydroponic culture