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. 2017 Mar;67(2):95-100.
doi: 10.1270/jsbbs.16115. Epub 2017 Mar 4.

Linkage mapping of Mungbean yellow mosaic India virus (MYMIV) resistance gene in soybean

Anita Rani  1 , Vineet Kumar  1 , B S Gill  2 , Pushpendra Rathi  3 , Shruti Shukla  1 , R K Singh  4 , S M Husain  1
Affiliations

Linkage mapping of Mungbean yellow mosaic India virus (MYMIV) resistance gene in soybean

Anita Rani et al. Breed Sci. 2017 Mar.

Abstract

Mungbean Yellow Mosaic India Virus (MYMIV) is one of the most prevalent pathogen that limits soybean production in India. In this study RILs derived from JS335, dominant but MYMIV susceptible variety and PI171443, donor of MYMIV resistance gene in most of the MYMIV resistant varieties released in India and F2 population derived from SL525, a resistant variety released for northern India and NRC101, a susceptible genotype were used to study the inheritance of MYMIV resistance and map the gene responsible for MYMIV resistance. F1s were found to be completely susceptible. F2:3 and RILs population segregated to fit a ratio of 1:2:1 and 1:1 indicating that a single recessive gene controlled resistance to MYMIV. BSA was performed using 144 polymorphic SSR markers. MYMIV resistance gene was mapped on chr 6 (LG C2) within a 3.5-cM genome region between two SSR markers GMAC7L and Satt322 whose size was estimated to be 77.115 kb (position of 12,259,594-12,336,709 bp). This is the first report on linkage mapping of MYMIV resistance gene in soybean. This will be helpful in breeding soybean varieties for resistance against MYMIV responsible for wide spread damage to soybean crop in India using Marker Assisted Selection.

Keywords: MYMIV; SSR; mapping; resistance gene; soybean.

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Figures

Fig. 1
Fig. 1
PCR amplification of RILs derived from JS335 × PI171443 with Satt322 (A) & GMAC7L (B). Lane marked JS represent JS335, lane marked PI represent PI171443 and lanes 1 to 26 (1–14 resistant plants and 15–26 susceptible plants) represent progeny rows and lane marked M represent molecular weight markers of 50 bp ladder.
Fig. 2
Fig. 2
The map positions and map orders of the MYMIV resistance gene, mapped with the primers of C2 linkage group on chr 6 in RILs of JS335 × PI171443 (A), the relevant segment of the soybean LG C2 from SoyBase (B) and the map position and map order of F2 population of SL525 × NRC101. R* is MYMIV resistance gene (C). Distances given are not to the scale.
See this image and copyright information in PMC

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