Comparative Study
doi: 10.1073/pnas.82.6.1781.
beta-Adrenergic agonist activity of a monoclonal anti-idiotypic antibody
- PMID: 2858851
- PMCID: PMC397356
- DOI: 10.1073/pnas.82.6.1781
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Comparative Study
beta-Adrenergic agonist activity of a monoclonal anti-idiotypic antibody
Proc Natl Acad Sci U S A.
1985 Mar.
Abstract
Hybridoma cells bearing monoclonal antibody against the beta-adrenergic ligand alprenolol were used as an immunogen to raise monoclonal anti-idiotypic antibodies. Of six anti-idiotypic antibodies, which inhibit ligand binding, three were able to recognize beta-adrenergic receptors. One of them, mAb2B4, an IgM that could be amplified into ascites, binds to the beta-adrenergic catecholamine receptors of intact epidermoid A431 cells and precipitates receptors solubilized from plasma membranes by digitonin. This antibody identifies the beta 2-adrenergic receptor of A431 cells as a single 55-kDa protein and stimulates adenylate cyclase activity. This stimulation is inhibited by the beta-adrenergic antagonist propranolol.
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