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. 2017:2017:8753815.
doi: 10.1155/2017/8753815. Epub 2017 May 15.

The Significant Pathways and Genes Underlying the Colon Cancer Treatment by the Traditional Chinese Medicine PHY906

Ziyuan Su  1 , Changyu Zhou  2 , Shaoyou Qin  2 , Erna Jia  2 , Zhenting Wu  3
Affiliations

The Significant Pathways and Genes Underlying the Colon Cancer Treatment by the Traditional Chinese Medicine PHY906

Ziyuan Su et al. Evid Based Complement Alternat Med. 2017.

Abstract

Background: We attempted to explore the molecular mechanism underlying PHY906 intervention of colon cancer.

Methods: The microarray data of tumors treated by PHY906 and PBS alone were downloaded from the public Gene Expression Omnibus database. The dataset was further analyzed for the differentially expressed genes (DEGs) and their related biological functions were analyzed, followed by function and pathways. Protein-protein interaction (PPI) network was constructed and the significant nodes were screened by network centralities and then the significant modules analysis. Besides, they were clustered and transcriptional factors (TFs) were predicted.

Results: The gene expression patterns changed induced by PHY906 treatment, including 414 upregulated and 337 downregulated DEGs. The biological process of response to steroid hormone stimulus and regulation of interferon-gamma production were significantly enriched by DEGs. Ezh2 (enhancer of zeste 2) was found to be the key node in PPI network. There are 12 significant TFs predicted for module 1 genes and 3 TFs for module 2 genes.

Conclusions: PHY906 treatment may function in protecting the epithelial barrier against tumor cell invasion by modulating IFN-γ level and mediating cancer cell death by activating the response to steroid hormone stimulus and activating the response to steroid hormone stimulus. E2f1, Hsfy2, and Nfyb may be therapeutic targets for colon cancer. PHY906 showed treatment efficacy in modulating cell apoptosis by intervening interferon-gamma production and response to steroid hormone stimulus. Ezh2 and its TFs such as E2f1, Hsfy2, and Nfyb may be the potential therapeutic targets for anticancer agents development.

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Figures

Figure 1
Figure 1
Volcano plot of the differential expression of genes.
Figure 2
Figure 2
The protein-protein interaction network for differentially expressed genes. The trigon represents upregulated genes and the square represents downregulated genes.
Figure 3
Figure 3
The significant modules and their related TFs. (a) Module 1; (b) module 2; TFs: transcriptional factors. The trigon represents upregulated genes and the square represents downregulated genes. The roundness means the transcriptional factors.
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