Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017:2017:6761719.
doi: 10.1155/2017/6761719. Epub 2017 May 17.

Optimization of Extraction Process for Antidiabetic and Antioxidant Activities of Kursi Wufarikun Ziyabit Using Response Surface Methodology and Quantitative Analysis of Main Components

Salamet Edirs  1   2   3 , Ablajan Turak  1   2 , Sodik Numonov  1   2   4 , Xuelei Xin  1   2 , Haji Akber Aisa  1   2
Affiliations

Optimization of Extraction Process for Antidiabetic and Antioxidant Activities of Kursi Wufarikun Ziyabit Using Response Surface Methodology and Quantitative Analysis of Main Components

Salamet Edirs et al. Evid Based Complement Alternat Med. 2017.

Abstract

By using extraction yield, total polyphenolic content, antidiabetic activities (PTP-1B and α-glycosidase), and antioxidant activity (ABTS and DPPH) as indicated markers, the extraction conditions of the prescription Kursi Wufarikun Ziyabit (KWZ) were optimized by response surface methodology (RSM). Independent variables were ethanol concentration, extraction temperature, solid-to-solvent ratio, and extraction time. The result of RSM analysis showed that the four variables investigated have a significant effect (p < 0.05) for Y1, Y2, Y3, Y4, and Y5 with R2 value of 0.9120, 0.9793, 0.9076, 0.9125, and 0.9709, respectively. Optimal conditions for the highest extraction yield of 39.28%, PTP-1B inhibition rate of 86.21%, α-glycosidase enzymes inhibition rate of 96.56%, and ABTS inhibition rate of 77.38% were derived at ethanol concentration 50.11%, extraction temperature 72.06°C, solid-to-solvent ratio 1 : 22.73 g/mL, and extraction time 2.93 h. On the basis of total polyphenol content of 48.44% in this optimal condition, the quantitative analysis of effective part of KWZ was characterized via UPLC method, 12 main components were identified by standard compounds, and all of them have shown good regression within the test ranges and the total content of them was 11.18%.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Response surface plot for the effects of investigated parameters on the EY.
Figure 2
Figure 2
Response surface plot for the effects of investigated parameters on the TPC.
Figure 3
Figure 3
Response surface plot for the effects of investigated parameters on the PTP-1B inhibition rate.
Figure 4
Figure 4
Response surface plot for the effects of investigated parameters on the α-glucosidase inhibition rate.
Figure 5
Figure 5
Response surface plot for the effects of investigated parameters on the ABTS inhibition rate.
Figure 6
Figure 6
UPLC chromatograms of the KWZ and mixed standard.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Olubomehin O. O., Abo K. A., Ajaiyeoba E. O. Alpha-amylase inhibitory activity of two Anthocleista species and in vivo rat model anti-diabetic activities of Anthocleista djalonensis extracts and fractions. Journal of Ethnopharmacology. 2013;146(3):811–814. doi: 10.1016/j.jep.2013.02.007. - DOI - PubMed
    1. China Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China, part 1. Beijing, China: China Medical Science Press; 2015.
    1. Xu X., Huangerhan B. Flora of Kazakh. part 4. Beijing, China: The Ethnic Publishing House; 2009.
    1. Ishanqulova B. A., Sharofova M. U. Vliyanie sbora "Fitobet" na techenie eksperimentalnovo sakharnogo diabeta. part 4. Dushanbe, Tajikistan: Vestnik Avisenni; 2004.
    1. Saleh N. A. M., El-karemy Z. A. R., Mansour R. M. A., Fayed A.-A. A. A chemosystematic study of some geraniaceae. Phytochemistry. 1983;22(11):2501–2505. doi: 10.1016/0031-9422(83)80148-4. - DOI

LinkOut - more resources