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. 2017:2017:7282653.
doi: 10.1155/2017/7282653. Epub 2017 May 17.

Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae

Zhenting Zhou  1 , Weichao Zhong  1   2 , Haiyan Lin  1 , Peng Huang  1 , Ning Ma  3 , Yuqing Zhang  3 , Chuying Zhou  1 , Yuling Lai  1 , Shaohui Huang  1 , Shiying Huang  1 , Lei Gao  1   4 , Zhiping Lv  1
Affiliations

Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae

Zhenting Zhou et al. Evid Based Complement Alternat Med. 2017.

Abstract

Alcoholic liver disease (ALD) is a series of abnormalities of liver function, including alcoholic steatosis, steatohepatitis, and cirrhosis. Hesperidin, the major constituent of flavanone in grapefruit, is proved to play a role in antioxidation, anti-inflammation, and reducing multiple organs damage in various animal experiments. However, the underlying mechanism of resistance to alcoholic liver injury is still unclear. Thus, we aimed to investigate the protective effects of hesperidin against ALD and its molecular mechanism in this study. We established an ALD zebrafish larvae model induced by 350 mM ethanol for 32 hours, using wild-type and transgenic line with liver-specific eGFP expression Tg (lfabp10α:eGFP) zebrafish larvae (4 dpf). The results revealed that hesperidin dramatically reduced the hepatic morphological damage and the expressions of alcohol and lipid metabolism related genes, including cyp2y3, cyp3a65, hmgcra, hmgcrb, fasn, and fads2 compared with ALD model. Moreover, the findings demonstrated that hesperidin alleviated hepatic damage as well, which is reflected by the expressions of endoplasmic reticulum stress and DNA damage related genes (chop, gadd45αa, and edem1). In conclusion, this study revealed that hesperidin can inhibit alcoholic damage to liver of zebrafish larvae by reducing endoplasmic reticulum stress and DNA damage, regulating alcohol and lipid metabolism.

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Figures

Figure 1
Figure 1
Experimental plan for zebrafish.
Figure 2
Figure 2
Alcoholic fatty liver model was established in zebrafish larvae. (a) Oil Red O staining for whole body of zebrafish larvae. (b) H&E staining for liver sections of zebrafish larvae. (c) Quantitative analysis for the results of Oil Red O staining (n = 20/group, three experiments). The data are presented as the means ± SEM (∗P < 0.05 versus control group).
Figure 3
Figure 3
Hesperidin reduced hepatic steatosis in zebrafish larvae induced by alcohol. (a) Oil Red O staining for whole body of zebrafish larvae. (b) Quantitative analysis for the results of Oil Red O staining (n = 20/group, three experiments). (c) Nile Red staining for intracellular lipid droplets in liver tissues of zebrafish larvae. (d) H&E staining for liver sections of zebrafish larvae. The data are presented as the means ± SEM (∗P < 0.05 versus control group; #P < 0.05 versus 350 mM EtOH group).
Figure 4
Figure 4
A model depicting the protective role of hesperidin in zebrafish larvae during acute alcoholic injury.
See this image and copyright information in PMC

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